Cell cycle regulation of pEg3, a new Xenopus protein kinase of the KIN1/PAR-1/MARK family
Cell cycle regulation of pEg3, a new Xenopus protein kinase of the KIN1/PAR-1/MARK family
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DOI：
10.1006/dbio.2001.0525
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发表时间：
2002-01-15
影响因子：
2.7
通讯作者：
Tassan, JP
中科院分区：
文献类型：
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作者：
Blot, J;Chartrain, I;Tassan, JP
我们报道了一种与KIN1/PAR-1/MARK家族成员相关的爪蟾蛋白激酶pEg3的表征。这个新出现的激酶家族的创始成员被证明参与了细胞极性和微管动力学和细胞骨架组织的建立。序列分析表明，pEg3和相关蛋白激酶在人类、小鼠和秀丽隐杆线虫中可能构成这个家族的一个不同的群体。pEg3由母体mRNA编码，在未受精卵中被聚腺苷化，在胚胎中被特异性地死烯化。除了表达增加外，我们还发现pEg3在卵母细胞成熟过程中被磷酸化。在爪蟾早期胚胎发生和同步培养的XL2细胞中，pEg3的磷酸化依赖于细胞周期。在胚胎中，pEg3的激酶活性与其磷酸化状态相关，并在有丝分裂期间达到最大。使用爪蟾卵提取物，我们证明磷酸化至少发生在激酶的非催化结构域，这表明该结构域可能对pEg3的功能很重要。(C) 2001 Elsevier Science。
We report the characterization of pEg3, a Xenopus protein kinase related to members of the KIN1/PAR-1/MARK family. The founding members of this newly emerging kinase family were shown to be involved in the establishment of cell polarity and both microtubule dynamic and cytoskeleton organization. Sequence analyses suggest that pEg3 and related protein kinases in human, mouse, and Caenorhabditis elegans might constitute a distinct group in this family. pEg3 is encoded by a maternal mRNA, polyadenylated in unfertilized eggs and specifically deadenylated in embryos. In addition to an increase in expression, we have shown that pEg3 is phosphorylated during oocyte maturation. Phosphorylation of pEg3 is cell cycle dependent during Xenopus early embryogenesis and in synchronized cultured XL2 cells. In embryos, the kinase activity of pEg3 is correlated to its phosphorylation state and is maximum during mitosis. Using Xenopus egg extracts we demonstrated that phosphorylation occurs at least in the noncatalytic domain of the kinase, suggesting that this domain might be important for pEg3 function. (C) 2001 Elsevier Science.