rax, a novel paired-type homeobox gene, shows expression in the anterior neural fold and developing retina
rax, a novel paired-type homeobox gene, shows expression in the anterior neural fold and developing retina
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DOI：
10.1073/pnas.94.7.3088
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发表时间：
1997-04-01
影响因子：
11.1
通讯作者：
Cepko, CL
中科院分区：
文献类型：
--
作者：
Furukawa, T;Kozak, CA;Cepko, CL
已经发现脊椎动物眼的发育需要编码同源结构域蛋白的几种基因的活性（Freund，C.，D.霍斯福德J.& McInnes. R. R.（1996年）摩尔Genet. 5，1471-1488）。这些基因中的一些或其部分在门中是高度保守的。在本文中，我们报告了一个新的同源异型盒基因，rax（视网膜和前神经折叠同源异型盒），其表达模式表明在眼睛发育中的重要作用的鉴定。预测的Rax的氨基酸序列包括具有配对型同源盒的蛋白质，以及在许多配对型同源盒基因中发现的八肽。此外，在Rax的C端，我们发现了一个15个氨基酸的结构域，我们将其命名为OAR结构域。该结构域也存在于其他几个同源异型盒基因中。在早期小鼠胚胎中，rax在前神经褶皱中表达，包括将产生腹侧前脑和视泡的区域。一旦视泡形成，rax的表达仅限于腹侧间脑和视泡。在后期阶段，rax表达仅在发育中的视网膜中发现。出生后，rax的表达仅限于视网膜内的增殖细胞区，并且随着增殖下降，表达逐渐减少。这些发现表明，pns是在早期发育过程中定义视野的分子之一，并且在视网膜细胞的增殖和/或分化中起作用。
Development of the vertebrate eye has been found to require the activity of several genes encoding homeodomain proteins (Freund, C,. Horsford, D. J. & McInnes. R. R. (1996) Hum. Mol. Genet. 5, 1471-1488). Some of these gents, or portions thereof, are highly conserved across phyla. In this paper, we report the identification of a novel homeobox gene, rax (retina and anterior neural fold homeobox), whose expression pattern suggests an important role in eye development. The predicted amino acid sequence of Rax comprises a protein with a paired-type homeobox, as well as the octapeptide that is found in many paired-type homeobox genes. In addition, in the C terminus of Rax, we found a 15-aa domain that we have named the OAR domain. This domain is also found in several other homeobox genes. In the early mouse embryo, rax is expressed in the anterior neural fold, including areas that will give rise to the ventral forebrain and optic vesicles. Once the optic vesicles form, rax expression is restricted to the ventral diencephalon and the optic vesicles. At later stages, rax expression is found only in the developing retina. After birth, the expression of rax is restricted to the zone of proliferating cells within the retina, and expression gradually decreases as proliferation declines. These findings suggest that pns is one of the molecules that define the eye field during early development and that it has a role in the proliferation and/or differentiation of retinal cells.