Functional and technological properties of exopolysaccharide producing autochthonous Lactobacillus plantarum strain AAS3 from dry fish based fermented food
Functional and technological properties of exopolysaccharide producing autochthonous Lactobacillus plantarum strain AAS3 from dry fish based fermented food
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DOI：
10.1016/j.lwt.2019.108387
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发表时间：
2019-11-01
影响因子：
6
通讯作者：
Khusro, Ameer
中科院分区：
文献类型：
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作者：
Aarti, Chirom;Khusro, Ameer
本研究旨在评价产生高效乳杆菌胞外多糖(EPS)的不同功能和工艺特性。首先，从Hentak中分离出10株原生益生菌，并对其产生EPS进行了筛选。其中，植物乳杆菌AAS3菌株是胞外多糖的最大产生菌(450.5+/-5.8 mg/L)，并用标准方法对其功能和工艺属性进行了进一步的评价。AAS3菌株在模拟胃液中(pH 2.0)仍能保持活力，细胞总数为2.4+/-0.06logCFU/mL。该菌株具有显著的β-半乳糖苷酶活性(18.3+/-0.6U/mL)、胆盐水解酶产量(3.32+/-0.5U/mL)和降胆固醇活性(41.6+/-0.8%)。菌株AAS3在清除羟基自由基方面表现出良好的抗氧化特性。该菌株耐受高浓度的氯化钠，发酵独特的碳水化合物，并产生重要的水解酶。此外，菌株AAS3具有良好的自溶特性(56.4+/-1.2%)、过氧化氢酶活力(22.34+/-0.3 AU)和亚硝酸盐产量(28.2+/-0.8 mm亚硝酸盐/mg干重)。最重要的是，该分离株不仅对体外溶血试验、DNA酶试验和明胶酶试验呈阴性结果，而且对常规抗生素试验也呈阴性反应。总之，显著的EPS产量、示范性的功能和技术特性以及安全性方面表明，植物乳杆菌菌株AAS3作为典型的辅助/起始培养物适合于食品加工行业。
The present study was aimed to evaluate the disparate functional and technological properties of exopoly-saccharide (EPS) producing potent Lactobacillus sp. Initially, 10 autochthonous probiotic bacteria were isolated from Hentak and screened for EPS production. Among them, Lactobacillus plantarum strain AAS3 was identified as maximum producer of EPS (450.5 +/- 5.8 mg/L) and assessed further for evaluating functional and technological attributes using standard methodologies. Strain AAS3 maintained its viability towards simulated gastric juice (pH 2.0) with total cell counts of 2.4 +/- 0.06 log cfu/mL. The isolate showed pronounced beta-galactosidase activity (18.3 +/- 0.6 U/mL), bile salt hydrolase production (3.32 +/- 0.5 U/mL), and cholesterol reducing property (41.6 +/- 0.8%). Propitious antioxidant traits of strain AAS3 were observed in terms of scavenging hydroxyl radicals. The isolate sustained high concentrations of NaCl, fermented distinctive carbohydrates, and produced important hydrolytic enzymes. Further, strain AAS3 displayed promising autolytic property (56.4 +/- 1.2%), catalase activity (22.34 +/- 0.3 AU), and nitrite production (28.2 +/- 0.8 mM nitrite/mg dry weight). Most importantly, the isolate showed not only negative results towards in vitro haemolytic, DNase, and gelatinase tests but also sensitivity to conventional antibiotics tested. In conclusion, pronounced EPS production, exemplary functional and technological traits, and safety aspects signify suitability of L. plantarum strain AAS3 in food processing industries as quintessential adjunct/starter culture.