Selective determination of quinones by high-performance liquid chromatography with on-line post column ultraviolet irradiation and peroxyoxalate chemiluminescence detection
Selective determination of quinones by high-performance liquid chromatography with on-line post column ultraviolet irradiation and peroxyoxalate chemiluminescence detection
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DOI：
10.1016/j.chroma.2006.07.078
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发表时间：
2006-11-10
影响因子：
4.1
通讯作者：
Kuroda, Naotaka
中科院分区：
文献类型：
--
作者：
Ahmed, Sameh;Fujii, Shuu;Kuroda, Naotaka
建立了在线紫外照射下过氧草酸盐化学发光（PO-CL）同时测定醌类化合物的高效液相色谱方法。醌类化合物[即1,2-萘醌，1,4-萘醌，9,10-蒽醌，9,10-菲醌]在254 nm， 15 W的紫外照射下生成过氧化氢和荧光产物，通过PO-CL检测测定。采用流动注射分析（FIA）系统，利用过氧化氢酶固定的酶柱反应器，证实了醌类化合物在在线紫外线照射下生成过氧化氢。此外，用LC-MS， IR和H-1 NMR证实了所产生的荧光团的结构。然后对紫外光照射条件和PO-CL检测条件进行了优化。四种醌类化合物在ODS柱上等压分离，分离时间为25 min。检测限（信噪比为3）分别为：1,2-萘醌6.0 pmol/支，1,4-萘醌4.4 pmol/支，9,10 -蒽醌0.2 pmol/支，9,10 -菲醌0.45 pmol/支。(c) 2006 Elsevier B.V.版权所有
A new HPLC method was developed for the simultaneous determination of quinones with peroxyoxalate chemiluminescence (PO-CL) detection following on-line UV irradiation. Quinones [i.e., 1,2-naphthoquinone, 1,4-naphthoquinone, 9,10-anthraquinone, 9,10-phenanthrenequinone] were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide and a fluorescent product that were determined via PO-CL detection. Generation of hydrogen peroxide from quinones with on-line UV irradiation was confirmed using flow injection analysis (FIA) system whereby incorporating an enzyme column reactor immobilized with catalase. Moreover, the structure of the produced fluorophore was confirmed using LC-MS, IR, and H-1 NMR. Afterwards, the conditions for UV irradiation and PO-CL detection were optimized. The separation of four quinones by HPLC was accomplished isocratically on an ODS column within 25 min. The detection limits (signal-to-noise ratio = 3) were 6.0 pmol/injection for 1,2-naphthoquinone, 4.4 pmol/injection for 1,4-naphthoquinone, 0.2 pmol/injection for 9, 10-anthraquinone, and 0.45 pmol/injection for 9, 10-phenanthrenequinone. (c) 2006 Elsevier B.V. All rights reserved.