Determination of phosphatidylethanol in blood from alcoholic males using high-performance liquid chromatography and evaporative light scattering or electrospray mass spectrometric detection
Determination of phosphatidylethanol in blood from alcoholic males using high-performance liquid chromatography and evaporative light scattering or electrospray mass spectrometric detection
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DOI：
10.1016/s0378-4347(97)00541-0
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发表时间：
1998-02-13
期刊：
影响因子：
--
通讯作者：
Odham, G
中科院分区：
文献类型：
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作者：
Gunnarsson, T;Karlsson, A;Odham, G
用高效液相色谱-蒸发光散射检测(ELSD)或电喷雾(ES)质谱仪测定了人血提取物中仅在乙醇存在下形成的病理性磷脂-磷脂酰乙醇(PEH)。使用二醇柱和正相二元梯度系统进行分离。ELSD可以检测到三名男性酗酒者的PEH浓度(15~1nmol/ml血液)下降，在戒酒期开始后3周，使用ES，检测到低至100pmol/ml血液的水平。PEH的分子种类与同一血样中的磷脂酰胆碱相似。该方法可快速定量和定性测定血液中的多糖核酸。ELSD的检出限为200pmol(约125 ng)，ES的检出限为140pmol(约100pg)，总进样量。(C)1998年爱思唯尔科学公司。
The 'pathologic' phospholipid, phosphatidylethanol (PEth), formed only in the presence of ethanol, was determined in extracts of human blood using high-performance liquid chromatography with evaporative light scattering detection (ELSD) or electrospray (ES) mass spectrometry. Separation was performed using a diol column and a normal-phase binary gradient system. Decreasing concentrations of PEth (15 to 1 nmol/ml blood) could be detected by ELSD in three male alcoholics, up to 3 weeks after the beginning of an alcohol-free period, Using ES, levels down to 100 pmol/ml blood was detected. The molecular species of PEth were similar to those of phosphatidylcholine found in the same blood sample. The method provides a rapid quantitative and qualitative determination of PEth in blood. The limits of detection were 200 pmol (approximate to 125 ng) using ELSD and 140 fmol (approximate to 100 pg) using ES, total amounts injected. (C) 1998 Elsevier Science B.V.