RNA-seq Insights Into the Impact of Alteromonas macleodii on Isochrysis galbana.
RNA-seq Insights Into the Impact of Alteromonas macleodii on Isochrysis galbana.
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RNA-seq 洞察 Macleodii 交替单胞菌对等鞭金藻的影响
DOI：
10.3389/fmicb.2021.711998
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发表时间：
2021
影响因子：
5.2
通讯作者：
Xu JL
中科院分区：
文献类型：
--
作者：
Cao JY;Wang YY;Wu MN;Kong ZY;Lin JH;Ling T;Xu SM;Ma SN;Zhang L;Zhou CX;Yan XJ;Xu JL
藻球细菌可能是影响藻类生长的关键生物因子。然而，关于等溶藻与其藻球细菌相互作用的研究还很有限。本研究表明，海洋异养细菌Alteromonas macleodii促进了galbana微藻的生长，并抑制了该微藻的非光化学猝灭（NPQ）和超氧化物歧化酶（SOD）活性。此外，我们通过研究与A. macleodii共培养时I. galbana的整个转录组如何变化来探索这一现象。在A. macleodii的存在下，与I. galbana光合作用、碳固定、氧化磷酸化、核糖体蛋白、生物合成酶和运输过程相关的转录本显著增加，这表明细菌的引入可能增加了碳化合物和其他类型生物分子的生产和运输。此外，从编码DNA修复酶、超氧化物歧化酶（SOD）和其他应激反应蛋白的转录本的消耗推断，转录组的变化在很大程度上与galbana的应激条件降低相对应。综上所述，a.m ecleodii的存在主要增强了galbana的光合作用和生物合成，并保护其免受胁迫，特别是氧化应激。固定有机碳的转移，也可能是其他类型的生物分子，在自养生物和异养生物之间可能发生在I. galbana中。macleodii培养。目前的工作为I. galbana与其异养细菌伴侣的互惠关系以及I. galbana- a中存在的互利关系的转录后果提供了新的见解。可以利用马氏藻来提高水产养殖藻类养殖系统的生产力和可持续性。
Phycospheric bacteria may be the key biological factors affecting the growth of algae. However, the studies about interaction between Isochrysis galbana and its phycospheric bacteria are limited. Here, we show that a marine heterotrophic bacterium, Alteromonas macleodii, enhanced the growth of I. galbana, and inhibited non-photochemical quenching (NPQ) and superoxide dismutase (SOD) activities of this microalgae. Further, we explored this phenomenon via examining how the entire transcriptomes of I. galbana changed when it was co-cultured with A. macleodii. Notable increase was observed in transcripts related to photosynthesis, carbon fixation, oxidative phosphorylation, ribosomal proteins, biosynthetic enzymes, and transport processes of I. galbana in the presence of A. macleodii, suggesting the introduction of the bacterium might have introduced increased production and transport of carbon compounds and other types of biomolecules. Besides, the transcriptome changed largely corresponded to reduced stress conditions for I. galbana, as inferred from the depletion of transcripts encoding DNA repair enzymes, superoxide dismutase (SOD) and other stress-response proteins. Taken together, the presence of A. macleodii mainly enhanced photosynthesis and biosynthesis of I. galbana and protected it from stress, especially oxidative stress. Transfer of fixed organic carbon, but perhaps other types of biomolecules, between the autotroph and the heterotroph might happen in I. galbana-A. macleodii co-culture. The present work provides novel insights into the transcriptional consequences of I. galbana of mutualism with its heterotrophic bacterial partner, and mutually beneficial associations existing in I. galbana-A. macleodii might be explored to improve productivity and sustainability of aquaculture algal rearing systems.