Localization of a second NM23 gene, NME2, to chromosome 17q21-q22.
Localization of a second NM23 gene, NME2, to chromosome 17q21-q22.
复制标题
第二个 NM23 基因 NME2 定位于染色体 17q21-q22。
作者：
D. Kelsell;D. Black;E. Solomon;N. Spurr
NM23是一种候选的肿瘤抑制蛋白，最近被鉴定为NDP激酶。NM23的表达与肿瘤细胞的转移潜能呈负相关。已经克隆了两个NM23基因，NME1和NME2，分别编码该激酶的A和B链。为了确定NME2基因在人类染色体上的位置，我们用聚合酶链式反应和Southern杂交相结合的方法，分析了啮齿动物-人类细胞系和含有17号染色体部分片段的杂交细胞系的DNA。NME2基因被定位在染色体17q21-q22区域，该区域与NME1基因定位的区域相同。该区域与早发性乳腺/卵巢基因座(BRCA1)有关，NME1等位基因缺失与结直肠癌的转移潜能有关。
NM23 is a candidate tumor suppressor protein and has recently been identified as an NDP kinase. The expression of NM23 is inversely related to the metastatic potential of tumor cells. Two NM23 genes, NME1 and NME2, that code for the A and B chains of the kinase, respectively, have been cloned. To determine the human chromosomal location of the NME2 gene, we have analyzed DNA from rodent-human cell lines and hybrid cell lines containing portions of chromosome 17 by a combination of PCR amplification and Southern hybridization. The NME2 gene was mapped to the chromosome region 17q21-q22, the same region in which the NME1 gene has been localized. This region is linked to the early onset breast/ovarian locus (BRCA1) and allelic deletions of NME1 have been associated with metastatic potential of colorectal carcinomas.