Endothelial Rho signaling is required for monocyte transendothelial migration
Endothelial Rho signaling is required for monocyte transendothelial migration
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DOI：
10.1016/s0014-5793(02)02643-1
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发表时间：
2002-04-24
期刊：
影响因子：
3.5
通讯作者：
Gerke, V
中科院分区：
文献类型：
--
作者：
Strey, A;Janning, A;Gerke, V
利用影响微血管内皮细胞中 Rho 活性的细菌毒素来阐明内皮 Rho 是否参与调节单核细胞跨培养内皮细胞单层的迁移。梭状芽胞杆菌 C3 外切酶使 Rho 失活，导致外周血单核细胞与内皮细胞的粘附增加，并降低单核细胞跨内皮细胞迁移的速率。细胞毒性坏死因子1介导的内皮Rho激活也降低了单核细胞迁移率，但不影响单核细胞-内皮粘附。因此，有效的白细胞外渗不仅需要迁移的白细胞内的 Rho 信号传导，还需要血管壁内皮衬里内的 Rho 信号传导。 (C) 2002 年欧洲生化学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Bacterial toxins affecting Rho activity in microvascular endothelial cells were employed to elucidate whether endothelial Rho participates in regulating the migration of monocytes across monolayers of cultured endothelial cells. Inactivation of Rho by the Clostridium C3 exoenzyme resulted in an increased adhesion of peripheral blood monocytes to the endothelium and a decreased rate of transendothelial monocyte migration. Cytotoxic necrotizing factor 1-mediated activation of endothelial Rho also reduced the rate of monocyte transmigration, but did not affect monocyte-endothelium adhesion. Thus, efficient leukocyte extravasation requires Rho signaling not only within the migrating leukocytes but also within the endothelial lining of the vessel wall. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.