Calcitonin gene-related peptide and Langerhans cell function.
Calcitonin gene-related peptide and Langerhans cell function.
复制标题
降钙素基因相关肽和朗格汉斯细胞功能。
DOI：
10.1038/jidsymp.1997.16
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Granstein,RD
中科院分区：
文献类型：
--
作者：
Torii,H;Hosoi,J;Asahina,A;Granstein,RD
形态学研究表明，朗格汉斯细胞 (LC) 经常与含有神经肽降钙素基因相关肽 (CGRP) 的表皮神经处于解剖位置。进行实验来检查 CGRP 是否调节 LC 功能。使用通过 Thy-1 和补体抗体处理而富集 LC 含量 (EC) 的新鲜制备的鼠表皮细胞群，研究了体外 LC 预暴露于 CGRP 对同种异体抗原呈递和特异性蛋白呈递至响应性 T 细胞系的影响。还检查了 EC 呈递肿瘤相关抗原以诱导和引发肿瘤免疫小鼠迟发型超敏反应 (DTH) 的能力。在这些测定中均观察到 CGRP 对抗原呈递的抑制作用。还进行了实验，检查皮内施用的 CGRP 调节对注射部位应用的半抗原诱导接触超敏反应 (CHS) 的能力。 CGRP 的施用导致注射部位免疫后 CHS 反应降低。新鲜制备的LC在接触CGRP后，细胞内cAMP显着增加，并且该过程可以被CGRP受体的特异性抑制剂抑制，证明了LC上CGRP受体的存在。 LC样系XS52中由LPS和GM-CSF诱导的B7-2表达以及腹膜巨噬细胞中由LPS诱导的B7-2表达被CGRP抑制。这种抑制可能部分解释了 CGRP 的抑制作用。总的来说，这些观察结果表明，神经对抗原呈递的调节可能发生在表皮中。Journal of Investigative Dermatology Symposium Proceedings 2:82–86, 1997
Morphologic studies have indicated that Langerhans cells (LC) are frequently in anatomic apposition with epidermal nerves containing the neuropeptide calcitonin gene-related peptide (CGRP). Experiments were undertaken to examine whether CGRP modulates LC function. The effect of pre-exposure of LC to CGRPin vitroon alloantigen presentation and specific protein presentation to a responsive T-cell line were studied using freshly prepared murine epidermal cell populations enriched for LC content (EC) by treatment with antibody to Thy-1 and complement. The ability of EC to present tumor-associated antigens for induction and elicitation of delayed-type hypersensitivity (DTH) in tumor-immune mice was also examined. Inhibitory effects of CGRP on antigen presentation were observed in each of these assays. Experiments were also performed examining the ability of intradermally administered CGRP to modulate induction of contact hypersensitivity (CHS) to a hapten applied at the injected site. Administration of CGRP led to a decrease in the CHS response after immunization at the site of injection. Intracellular cAMP was significantly increased in freshly prepared LC after exposure to CGRP, and this process could be inhibited by a specific inhibitor of the CGRP receptor, demonstating the existence of CGRP receptors on LC. B7-2 expression induced by LPS and GM-CSF in the LC-like line XS52, and by LPS in peritoneal macrophages, was suppressed by CGRP. This suppression may account, in part, for the inhibitory effect of CGRP. As a whole, these observations suggest that regulation of antigen presentation by nerves may occur in the epidermis.Journal of Investigative Dermatology Symposium Proceedings 2:82–86, 1997