Association of Ferredoxin:NADP(+) oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii.
Association of Ferredoxin:NADP(+) oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii.
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DOI：
10.1007/s11120-017-0408-5
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发表时间：
2017-12
影响因子：
3.7
通讯作者：
Hippler M
中科院分区：
文献类型：
--
作者：
Mosebach L;Heilmann C;Mutoh R;Gäbelein P;Steinbeck J;Happe T;Ikegami T;Hanke G;Kurisu G;Hippler M
铁氧还蛋白(FDX)和FDX：NADP+氧化还原酶(FNR)是光系统I(PSI)下游电子传递的关键环节。FNR在类囊体膜上的动态募集被认为是决定光合作用衍生电子命运的一种潜在机制。在这项研究中，我们研究了FNR与莱茵衣藻光合作用装置结合的功能重要性。基于NADP+光还原测量和核磁共振化学位移扰动分析的体外分析表明，与FDX2相比，FNR优先与FDX1相互作用。值得注意的是，FNR与PSI超复合物的结合进一步增强了FDX1比FDX2的这种偏好，表明FNR有可能将电子引导到不同的路线。NADP+光还原分析和免疫印迹表明，在没有质子梯度调节5(PGR5)和/或类质子梯度调节5(PGRL1)的情况下，FNR与包括PSI超复合体在内的类囊体膜的结合受到损害，这意味着这两种蛋白都直接或间接地促进了FNR向类囊体膜的募集。通过体内吸收光谱和免疫印迹分析，在缺少PGR5和/或PGRL1的情况下，PSI是强光胁迫下光损伤的主要目标。缺氧保存了PSI的活性，表明对O2的电子供给量增加是PSI失活和降解的来源。这些发现建立了对PGR5/PGRL1基因敲除相关表型的另一种观点，并可能将FNR与调节莱茵哈迪尔乳杆菌的光合作用电子传递和PSI光保护联系起来。
Ferredoxins (FDX) and the FDX:NADP+ oxidoreductase (FNR) represent a key junction of electron transport downstream of photosystem I (PSI). Dynamic recruitment of FNR to the thylakoid membrane has been considered as a potential mechanism to define the fate of photosynthetically derived electrons. In this study, we investigated the functional importance of the association of FNR with the photosynthetic apparatus in Chlamydomonas reinhardtii. In vitro assays based on NADP+ photoreduction measurements as well as NMR chemical shift perturbation analyses showed that FNR preferentially interacts with FDX1 compared to FDX2. Notably, binding of FNR to a PSI supercomplex further enhanced this preference for FDX1 over FDX2, suggesting that FNR is potentially capable of channelling electrons towards distinct routes. NADP+ photoreduction assays and immunoblotting revealed that the association of FNR with the thylakoid membrane including the PSI supercomplex is impaired in the absence of Proton Gradient Regulation 5 (PGR5) and/or Proton Gradient Regulation 5-Like photosynthetic phenotype 1 (PGRL1), implying that both proteins, directly or indirectly, contribute to the recruitment of FNR to the thylakoid membrane. As assessed via in vivo absorption spectroscopy and immunoblotting, PSI was the primary target of photodamage in response to high-light stress in the absence of PGR5 and/or PGRL1. Anoxia preserved the activity of PSI, pointing to enhanced electron donation to O2 as the source of the observed PSI inactivation and degradation. These findings establish another perspective on PGR5/PGRL1 knockout-related phenotypes and potentially interconnect FNR with the regulation of photosynthetic electron transport and PSI photoprotection in C. reinhardtii.
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DOI：
10.1073/pnas.0503952102
发表时间：
2005-07-05
影响因子：
11.1
作者：
Avenson, TJ;Cruz, JA;Kramer, DM
通讯作者：
Kramer, DM
影响因子：
4.3
作者：
Alric, Jean
通讯作者：
Alric, Jean
影响因子：
3.5
作者：
CLARK, RD;HAWKESFORD, MJ;HIND, G
通讯作者：
HIND, G
影响因子：
11.6
作者：
Dang, Kieu-Van;Plet, Julie;Peltier, Gilles
通讯作者：
Peltier, Gilles
影响因子：
64.8
作者：
Iwai, Masakazu;Takizawa, Kenji;Minagawa, Jun
通讯作者：
Minagawa, Jun