INCREASED BONE-MARROW PRODUCTION OF GRANULOCYTES AND MONONUCLEAR PHAGOCYTES INDUCED BY MYCOBACTERIAL ADJUVANTS - IMPROVED RECOVERY OF LEUKOPOIESIS IN MICE AFTER CYCLOPHOSPHAMIDE TREATMENT
INCREASED BONE-MARROW PRODUCTION OF GRANULOCYTES AND MONONUCLEAR PHAGOCYTES INDUCED BY MYCOBACTERIAL ADJUVANTS - IMPROVED RECOVERY OF LEUKOPOIESIS IN MICE AFTER CYCLOPHOSPHAMIDE TREATMENT
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DOI：
10.1128/iai.20.1.58-65.1978
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发表时间：
1978-01-01
影响因子：
3.1
通讯作者：
SHIFRINE, M
中科院分区：
文献类型：
--
作者：
BUHLES, WC;SHIFRINE, M
本文研究了完全弗氏佐剂（CFA）或牛分枝杆菌卡介苗（BCG）对环磷酰胺（CP）处理小鼠白细胞生成和白细胞恢复的影响。CFA皮下注射或i. p.导致血液粒细胞和单核细胞计数增加，骨髓粒细胞和单核吞噬细胞祖细胞数量增加，以及血清中造血集落刺激因子增加。24小时内对诱导的无菌i. p.炎症（巯基乙酸盐）的定量细胞反应通过s.c.终审法院在皮下注射CFA的小鼠中，在给予250 mg/kg剂量的CP后，血液粒细胞计数以及腹膜内粒细胞和巨噬细胞对巯基乙酸盐的反应比对照组恢复得更快。CFA处理的小鼠持续保持血液粒细胞和单核细胞计数比对照组高1.3至4倍，持续2周，同时每隔一天接受75 mg CP/kg。腹腔注射CFA预处理的小鼠在250 mg/kg CP注射后，培养物中的骨髓集落形成单位数量较高，血清集落刺激因子水平较高。同样，BCG导致培养物中骨髓集落形成单位增加，血清集落刺激因子增加，CP注射后腹膜炎症反应更快恢复。分枝杆菌佐剂加速CP治疗后白细胞生成功能的恢复。可能存在这样的佐剂在免疫抑制小鼠中提供针对感染的非特异性保护的机制。
The effects of complete Freund adjuvant (CFA) or Mycobacterium bovis BCG on leukopoiesis and on leukopoietic recovery from cyclophosphamide [CP] treatment in mice was studied. CFA injected s.c. or i.p. resulted in increased blood granulocyte and monocyte counts, increased numbers of bone marrow granulocyte and mononuclear phagocyte progenitors, and increased hematopoietic colony-stimulating factor in the serum. The quantitative cellular response within 24 h to an induced sterile i.p. inflammation (thioglycolate) was augmented by s.c. CFA. In mice given CFA s.c., blood granulocyte counts, as well as the peritoneal granulocyte and macrophage response to i.p. thioglycolate, recovered more quickly than did those of the controls after a 250-mg/kg dose of CP. CFA-treated mice consistently maintained blood granulocyte and monocyte counts 1.3- to 4-fold higher than those of the controls for 2 wk while receiving 75 mg of CP/kg every other day. Mice pretreated with CFA i.p. had higher numbers of bone marrow colony-forming units in culture and higher levels of serum colony-stimulating factor after 250-mg/kg injections of CP. Similarly, BCG resulted in increased bone marrow colony-forming units in culture, increased serum colony-stimulati factor and a faster return of the peritoneal inflammatory response after CP injection. Mycobacterial adjuvants accelerate recovery of leukopoietic functions after CP treatment. There may be a mechanism whereby such adjuvants afford nonspecific protection against infection in immunosuppressed mice.