Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology
Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology
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DOI：
10.1007/978-1-60761-789-1_1
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Tosi, Sabrina
中科院分区：
文献类型：
--
作者：
Garimberti, Elisa;Tosi, Sabrina
荧光原位杂交（FISH）广泛用于中期和间期细胞中目标染色体上基因和特定基因组区域的定位。FISH的应用并不局限于基因定位或人类疾病中基因重排的研究。事实上，FISH越来越多地用于探索各种生物体中的基因组组织，并扩展到动物和植物生物学的研究。本文介绍了FISH技术应用于中期和间期染色体研究的原理和基本方法。
Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and specific genomic regions on target chromosomes, both in metaphase and interphase cells. The applications of FISH arc not limited to gene mapping or the study of genetic rearrangements in human diseases. Indeed, FISH is increasingly used to explore the genome organization in various organisms and extends to the study of animal and plant biology. We have described the principles and basic methodology of FISH to be applied to the study of metaphase and interphase chromosomes.