A cell-specific enhancer that specifies lin-3 expression in the C-elegans anchor cell for vulval development
A cell-specific enhancer that specifies lin-3 expression in the C-elegans anchor cell for vulval development
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DOI：
10.1242/dev.00924
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发表时间：
2004-01-01
期刊：
影响因子：
4.6
通讯作者：
Sternberg, PW
中科院分区：
文献类型：
--
作者：
Hwang, BJ;Sternberg, PW
在秀丽隐杆线虫外阴发育过程中，体细胞性腺中的锚细胞（AC）表达lin-3，激活外阴前体细胞（VPC）中的EGF受体信号通路，从而诱导和形成VPC。先前对 lin-3 突变体和转基因表达的研究表明，AC 中 LIN-3 的水平必须受到精确调节才能实现外阴的正常发育。为了了解 lin-3 在 AC 中如何表达，我们鉴定了一个 59 bp lin-3 增强子，足以仅在 AC 中激活 lin-3 转录。该增强子包含两个 E-box 元件和一个 FTZ-F1 核激素受体 (NHR) 结合位点，该结合位点在无外阴突变体 lin-3(e1417) 中发生突变。诱变研究表明，E-box 和 NHR 结合位点都是 AC 中表达 lin-3 所必需的。体外 DNA 结合研究和体内功能测定表明，不同的反式作用因子，包括 E 蛋白/Daughterless 同源物 HLH-2 和未鉴定的核激素受体，是 AC 中 lin-3 转录所必需的，因此参与外阴发育。
During C. elegans vulval development, the anchor cell (AC) in the somatic gonad expresses lin-3, activating the EGF receptor signaling pathway in vulval precursor cells (VPCs) and thereby inducing and patterning VPCs. Previous studies with lin-3 mutants and transgene expression have revealed that the level of LIN-3 in the AC must be precisely regulated for proper vulval development. To understand how lin-3 expression is achieved in the AC, we identified a 59 bp lin-3 enhancer sufficient to activate lin-3 transcription solely in the AC. The enhancer contains two E-box elements, and one FTZ-F1 nuclear hormone receptor (NHR) binding site that is mutated in a vulvaless mutant, lin-3(e1417). Mutagenesis studies show that both E-boxes and the NHR binding site are necessary to express lin-3 in the AC. In vitro DNA-binding studies and in vivo functional assays indicate that distinct trans-acting factors, including the E-protein/Daughterless homolog HLH-2 and unidentified nuclear hormone receptor(s), are necessary for lin-3 transcription in the AC and thus are involved in vulval development.