P34CDC2 IS LOCATED IN BOTH NUCLEUS AND CYTOPLASM - PART IS CENTROSOMALLY ASSOCIATED AT G2/M AND ENTERS VESICLES AT ANAPHASE
P34CDC2 IS LOCATED IN BOTH NUCLEUS AND CYTOPLASM - PART IS CENTROSOMALLY ASSOCIATED AT G2/M AND ENTERS VESICLES AT ANAPHASE
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DOI：
10.1002/j.1460-2075.1989.tb08581.x
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发表时间：
1989-12-20
期刊：
影响因子：
11.4
通讯作者：
BORNENS, M
中科院分区：
文献类型：
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作者：
BAILLY, E;DOREE, M;BORNENS, M
cdc2+基因产物p34cdc2通过免疫细胞化学方式存在于人细胞的细胞核和细胞质中。它在细胞质中均匀分布，在细胞核中不规则分布。p34cdc2的一部分与中心体有关，中心体染色在细胞周期晚期和有丝分裂开始时增加。细胞分离证实了这种分布，这也表明p34cdc2在分离的中心体和triton不溶性部分中发现了较慢的迁移形式。我们提出p34cdc2蛋白激酶的一个作用是修饰中心体，导致有丝分裂纺锤体的形成。在后期，p34cdc2与细胞中间重组核之间的囊泡相关。p13suc1也有类似的位置，我们认为泡定位在有丝分裂结束时p34cdc2激酶失活中起作用。
The cdc2+ gene product p34cdc2 is located immunocytochemically in both the nucleus and cytoplasm of human cells. It is uniformly distributed throughout the cytoplasm and is irregularly distributed in the nucleus. Part of p34cdc2 is associated with the centrosome and centrosomal staining increases late in the cell cycle and at the onset of mitosis. This distribution is corroborated by cell fractionation which also indicates that slower migrating forms of p34cdc2 are found in isolated centrosomes and in Triton-insoluble fractions. We propose that one role of the p34cdc2 protein kinase is to modify the centrosome bringing about formation of the mitotic spindle. At anaphase p34cdc2 becomes associated with vesicles in the middle of the cell between the reforming nuclei. A similar location is found for p13suc1 and we suggest that the vesicular localization plays a role in p34cdc2 kinase inactivation at the end of mitosis.