Structural Insights into Nuclear p e-mRNA Splicing in Higher Eukaryotes
Structural Insights into Nuclear p e-mRNA Splicing in Higher Eukaryotes
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DOI：
10.1101/cshperspect.a032417
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发表时间：
2019-11-01
影响因子：
7.2
通讯作者：
Luehrmann, Reinhard
中科院分区：
文献类型：
--
作者：
Kastner, Berthold;Will, Cindy L.;Luehrmann, Reinhard
剪接体是一种高度复杂的、动态的核糖核蛋白分子机器，它经历了许多结构和组成的重排，导致其活性位点的形成。冷冻电子显微镜（cryo-EM）的最新进展提供了大量关于剪接体内部工作的近原子结构信息。借助以前的生物化学，结构和功能研究，cryo-EM已经证实或提供了大多数剪接体功能的流行模型的结构基础，但同时允许新的见解剪接催化和剪接体的有趣的动力学。前体mRNA剪接的机制在人类和酵母之间是高度保守的，但人类剪接体的组成动力学和核糖核蛋白（RNP）重塑更为复杂。在这里，我们总结了最近的进展，我们了解的分子结构的人类剪接体，突出人类和酵母剪接机之间的差异。
The spliceosome is a highly complex, dynamic ribonucleoprotein molecular machine that undergoes numerous structural and compositional rearrangements that lead to the formation of its active site. Recent advances in cyroelectron microscopy (cryo-EM) have provided a plethora of near-atomic structural information about the inner workings of the spliceosome. Aided by previous biochemical, structural, and functional studies, cryo-EM has confirmed or provided a structural basis for most of the prevailing models of spliceosome function, but at the same time allowed novel insights into splicing catalysis and the intriguing dynamics of the spliceosome. The mechanism of pre-mRNA splicing is highly conserved between humans and yeast, but the compositional dynamics and ribonucleoprotein (RNP) remodeling of the human spliceosome are more complex. Here, we summarize recent advances in our understanding of the molecular architecture of the human spliceosome, highlighting differences between the human and yeast splicing machineries.