Quantitative analyses of interactions between SpoVG and RNA/DNA.
Quantitative analyses of interactions between SpoVG and RNA/DNA.
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SpoVG 和 RNA/DNA 之间相互作用的定量分析。
DOI：
10.1101/2023.02.06.527361
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Stevenson,Brian
中科院分区：
文献类型：
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作者：
Saylor,TimothyC;Savage,ChristinaR;Krusenstjerna,AndrewC;Jusufovic,Nerina;Zückert,WolframR;Brissette,CatherineA;Motaleb,Md;Schlax,PaulaJ;Stevenson,Brian
伯氏疏螺旋体SpoVG蛋白先前已被发现是DNA和RNA结合蛋白。为了帮助阐明配体基序，测量并比较了许多RNA、ssDNA和dsDNA的亲和力。研究中使用的基因座为espoVG、glpFKD、erpAB、bb 0242、flaB和dospAB，特别关注mRNA的5′端非翻译部分。结合和竞争实验表明，spoVGmRNA的5′端亲和力最高，而laBmRNA的5 ′端亲和力最低。spoVGRNA和ssDNA序列的突变研究表明，SpoVG-nucleic acid complex的形成不完全依赖于序列或结构。此外，在ssDNA中将尿嘧啶替换为胸腺嘧啶并不影响蛋白质-核酸复合物的形成。
TheBorrelia burgdorferiSpoVG protein has previously been found to be a DNA- and RNA-binding protein. To aid in the elucidation of ligand motifs, affinities for numerous RNAs, ssDNAs, and dsDNAs were measured and compared. The loci used in the study werespoVG, glpFKD, erpAB, bb0242, flaB,andospAB, with particular focus on the untranslated 5′ portion of the mRNAs. Performing binding and competition assays yielded that the 5′ end ofspoVGmRNA had the highest affinity while the lowest observed affinity was to the 5’ end offlaBmRNA. Mutagenesis studies ofspoVGRNA and ssDNA sequences suggested that the formation of SpoVG-nucleic acid complexes are not entirely dependent on either sequence or structure. Additionally, exchanging uracil for thymine in ssDNAs did not affect protein-nucleic acid complex formation.