Ionization and fragmentation of N-linked glycans as silver adducts by electrospray mass spectrometry
Ionization and fragmentation of N-linked glycans as silver adducts by electrospray mass spectrometry
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DOI：
10.1002/rcm.1815
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发表时间：
2005-01-01
影响因子：
2
通讯作者：
Harvey, DJ
中科院分区：
文献类型：
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作者：
Harvey, DJ
通过向电喷雾溶剂中加入银，从牛核糖核酸酶、鸡蛋糖蛋白、牛胎球蛋白和猪甲状腺球蛋白中获得的中性高甘露糖、杂合和复合N-连接聚糖通过电喷雾产生[M+Ag]（+）离子。产生了单电荷和双电荷离子，但由于信号在两种银同位素之间分裂，灵敏度不如先前报道的钠加合物高。碰撞诱导解离（CID）光谱主要由糖苷裂解产生的离子，主要是B-和Y-型。涉及B和Y裂解的内部裂解离子非常突出，但交叉环片段通常丰度非常低或不存在。银在切割糖苷键方面非常有效，以至于光谱往往在组成单糖的大多数可能组合中含有糖苷离子。版权所有（C）2005约翰威利父子有限公司。
[M+Ag](+) ions were produced by electrospray from neutral high-mannose, hybrid and complex N-linked glycans obtained from bovine ribonuclease, chicken egg glycoproteins, bovine fetuin and porcine thyroglobulin by the addition of silver nitrate to the electrospray solvent. Both singly and doubly charged ions were produced but, as the signals were split between the two silver isotopes, sensitivity was not as high as with the sodium adducts reported earlier. Collision-induced dissociation (CID) spectra were dominated by ions produced by glycosidic cleavages, mainly of the B- and Y-type. Internal cleavage ions involving both B and Y cleavages were very prominent but cross-ring fragments were generally of very low abundance or absent. Silver was very efficient at cleaving the glycosidic bonds, so much so that spectra tended to contain glycosidic ions at most possible combinations of the constituent monosaccharides. Copyright (C) 2005 John Wiley Sons, Ltd.