Production of polymalic acid and malic acid by Aureobasidium pullulans fermentation and acid hydrolysis
Production of polymalic acid and malic acid by Aureobasidium pullulans fermentation and acid hydrolysis
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DOI：
10.1002/bit.24876
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发表时间：
2013-08
影响因子：
3.8
通讯作者：
X. Zou;Yipin Zhou;Shangtian Yang
中科院分区：
文献类型：
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作者：
X. Zou;Yipin Zhou;Shangtian Yang
苹果酸是一种广泛应用于食品工业的二元酸，也是一种潜在的C4平台化学品，可由生物质生产。然而，由于最终产物的抑制，微生物发酵直接生产苹果酸的产量、效价和生产率都很低。开发了一种以聚马来酸(PMA)发酵后酸解为原料生产苹果酸的新工艺。首先，对一株产PMA的出芽短梗霉ZX-10进行了筛选和分离。该菌在搅拌槽式生物反应器中发酵，其主要发酵产物聚甲基丙烯酸甲酯的效价相当于苹果酸87.6g/L，发酵产率为0.61g/L h。在纤维床生物反应器中固定化细胞的补料分批发酵的最高产物效价为144.2 g/L，产率为0.74g/L h，发酵产物经IRA-900阴离子交换树脂吸附纯化，∼纯度为100%，回收率为84%。以PMA为原料，在85℃下用2M硫酸进行水解制得纯苹果酸，反应符合一级反应动力学。该工艺为PMA和苹果酸的生产提供了一条高效、经济的途径，具有很好的工业应用前景。生物技术。比昂斯。2013；110：2105-2113。©2013 Wiley期刊，Inc.
Malic acid is a dicarboxylic acid widely used in the food industry and also a potential C4 platform chemical that can be produced from biomass. However, microbial fermentation for direct malic acid production is limited by low product yield, titer, and productivity due to end‐product inhibition. In this work, a novel process for malic acid production from polymalic acid (PMA) fermentation followed by acid hydrolysis was developed. First, a PMA‐producing Aureobasidium pullulans strain ZX‐10 was screened and isolated. This microbe produced PMA as the major fermentation product at a high‐titer equivalent to 87.6 g/L of malic acid and high‐productivity of 0.61 g/L h in free‐cell fermentation in a stirred‐tank bioreactor. Fed‐batch fermentations with cells immobilized in a fibrous‐bed bioreactor (FBB) achieved the highest product titer of 144.2 g/L and productivity of 0.74 g/L h. The fermentation produced PMA was purified by adsorption with IRA‐900 anion‐exchange resins, achieving a ∼100% purity and a high recovery rate of 84%. Pure malic acid was then produced from PMA by hydrolysis with 2 M sulfuric acid at 85°C, which followed the first‐order reaction kinetics. This process provides an efficient and economical way for PMA and malic acid production, and is promising for industrial application. Biotechnol. Bioeng. 2013; 110: 2105–2113. © 2013 Wiley Periodicals, Inc.