The Arabidopsis peroxisome division mutant pdd2 is defective in the DYNAMIN-RELATED PROTEIN3A (DRP3A) gene
The Arabidopsis peroxisome division mutant pdd2 is defective in the DYNAMIN-RELATED PROTEIN3A (DRP3A) gene
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DOI：
10.4161/psb.4.6.8699
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Hu, Jianping
中科院分区：
文献类型：
--
作者：
Aung, Kyaw;Hu, Jianping
在植物中，过氧化物酶体的分裂由几类蛋白质介导，包括过氧化物酶11（PEX11）、裂变1（FIS 1）和蛋白相关蛋白3（DRP 3）。DRP3A和DRP3B是两个同源的动力蛋白相关蛋白，在过氧化物酶体和线粒体的分裂中发挥重叠的作用，其中DRP3A在过氧化物酶体分裂中比DRP3B执行更强的功能。在这里，我们报告的过氧化物酶体分裂缺陷2（pdd 2）突变体，后来被证明是另一个drp3A等位基因的鉴定和表征。pdd 2突变体产生截短的DRP3A蛋白，并表现出淡绿色和生长迟缓的表型。有趣的是，这个突变体显示更强的过氧化物酶体分裂缺陷在根细胞比在叶叶肉细胞。我们的数据表明，保留在pdd2的部分GTdR效应域可能有助于这种突变体的独特的突变表型。
In plants, the division of peroxisomes is mediated by several classes of proteins, including PEROXIN11 (PEX11), FISSION1 (FIS1) and DYNAMIN-RELATED PROTEIN3 (DRP3). DRP3A and DRP3B are two homologous dynamin-related proteins playing overlapping roles in the division of both peroxisomes and mitochondria, with DRP3A performing a stronger function than DRP3B in peroxisomal fission. Here, we report the identification and characterization of the peroxisome division defective 2 (pdd2) mutant, which was later proven to be another drp3A allele. The pdd2 mutant generates a truncated DRP3A protein and exhibits pale green and retarded growth phenotypes. Intriguingly, this mutant displays much stronger peroxisome division deficiency in root cells than in leaf mesophyll cells. Our data suggest that the partial GTPase effector domain retained in pdd2 may have contributed to the distinct mutant phenotype of this mutant.