DENOVO METHYLATION AND EXPRESSION OF RETROVIRAL GENOMES DURING MOUSE EMBRYOGENESIS
DENOVO METHYLATION AND EXPRESSION OF RETROVIRAL GENOMES DURING MOUSE EMBRYOGENESIS
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DOI：
10.1038/298623a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
JAENISCH, R
中科院分区：
文献类型：
--
作者：
JAHNER, D;STUHLMANN, H;JAENISCH, R
通过显微注射克隆DNA将逆转录病毒基因组导入小鼠受精卵，或通过感染Moloney小鼠白血病病毒(M-MuLV)将逆转录病毒基因组导入植入前桑蚕期小鼠胚胎，由于发生了新甲基化并阻断了表达。植入后的小鼠胚胎感染病毒后，未见病毒表达受抑制和结节新甲基化现象。高效的新甲基化活性可能是小鼠早期胚胎基因调控的一个重要特征。
Retrovirus genomes introduced into mouse zygotes by microinjection of cloned DNA, or into morula stage pre-implantation mouse embryos by infection with Moloney murine leukaemia virus (M-MuLV), becamede novomethylated and were blocked in expression. No restriction of virus expression and node novomethylation were observed when post-implantation mouse embryos were infected with virus. Efficientde novomethylation activity may be an important characteristic of gene regulation in early mouse embryos.