Daily variation in global and local DNA methylation in mouse livers.
Daily variation in global and local DNA methylation in mouse livers.
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小鼠肝脏中整体和局部 DNA 甲基化的每日变化
DOI：
10.1371/journal.pone.0118101
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Xia L;Ma S;Zhang Y;Wang T;Zhou M;Wang Z;Zhang J
DNA甲基化是最具特征的表观遗传修饰之一，具有重要的生物学相关性。在这里，我们发现小鼠肝脏中的总体DNA甲基化水平显示出每日变化，其中高峰阶段发生在一天结束时，最低水平发生在一天开始时的光-暗或暗-暗循环中。典型的重复序列长穿插核苷酸元件-1 （LINE-1）具有与全局DNA相似的甲基化节律。DNA甲基转移酶3A （DNMT3A）和s -腺苷蛋氨酸（SAM）与s -腺苷同型半胱氨酸（SAH）之比对总体DNA甲基化产生相对正向的日变化，SAM与SAH之比的暂时变化对DNA甲基化水平没有影响。Per1-/- per2 -/-双敲除小鼠整体DNA甲基化节律丧失，DNA甲基化水平升高，这与Dnmt3a mRNA水平及其节律变化一致。我们的研究结果表明，全球DNA甲基化的每日变化与Dnmt3a表达的变化有关，而不是SAM与SAH的比例。
DNA methylation is one of the best-characterized epigenetic modifications and has an important biological relevance. Here we showed that global DNA methylation level in mouse livers displayed a daily variation where the peak phases occurred during the end of the day and the lowest level at the beginning of the day in the light-dark or dark-dark cycles. Typical repeat sequence long interspersed nucleotide element-1 (LINE-1) had a similar methylation rhythm to global DNA. DNA methyltransferase 3A (DNMT3A) and ratio of S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH) brought a relative forward daily variation to global DNA methylation, and the temporary change in ratio of SAM to SAH had no influence on the DNA methylation level. The rhythm of global DNA methylation was lost and DNA methylation level was increased in Per1-/-Per2-/- double knockout mice, which were in accordance with changes of Dnmt3a mRNA levels and its rhythm. Our results suggest that the daily variation in global DNA methylation was associated with the change of Dnmt3a expression rather than ratio of SAM to SAH.
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