Yeast Pho85 kinase is required for proper gene expression during the diauxic shift
Yeast Pho85 kinase is required for proper gene expression during the diauxic shift
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DOI：
10.1002/yea.1138
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发表时间：
2004-08
期刊：
影响因子：
2.6
通讯作者：
M. Nishizawa;Y. Katou;K. Shirahige;A. Toh-e
中科院分区：
文献类型：
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作者：
M. Nishizawa;Y. Katou;K. Shirahige;A. Toh-e
芽殖酵母酿酒酵母（Saccharomyces cerevisiae）在环境营养条件改变时会改变其基因表达谱。蛋白激酶包括环AMP依赖性激酶、Snf1和Tor激酶在此过程中发挥重要作用。Pho85激酶是酵母细胞周期蛋白依赖性激酶家族的一员，参与磷酸盐代谢和碳水化合物储备的调节，因此被认为是一种营养传感激酶。培养基中的葡萄糖耗尽后，酵母细胞经历二次代谢转变，同时伴有碳代谢途径转变、线粒体功能刺激以及核糖体生物发生和蛋白质合成下调。我们分析了pho85Δ突变对该过程中基因表达谱的影响，以研究Pho85激酶是否参与酵母二次氧化。我们发现，在没有PHO85的情况下，大多数线粒体基因没有被适当地诱导，蛋白酶体相关基因和伴侣蛋白基因被更多地抑制，并且，当培养基中仍然存在葡萄糖时，参与核糖体生物合成的某些类别的基因（核糖体蛋白和rRNA加工基因）被抑制，而那些参与细胞异生和乙醛酸循环的基因被诱导。我们还发现PHO85是几个金属传感器基因及其调控基因正常表达所必需的。这些结果表明，PHO85是必要的适当的发病负责的二次移位的基因的表达谱的变化。版权所有© 2004年约翰威利父子有限公司。
The budding yeast Saccharomyces cerevisiae changes its gene expression profile when environmental nutritional conditions are changed. Protein kinases including cyclic AMP‐dependent kinase, Snf1 and Tor kinases play important roles in this process. Pho85 kinase, a member of the yeast cyclin‐dependent kinase family, is involved in the regulation of phosphate metabolism and reserve carbohydrates, and thus is implicated to function as a nutrient‐sensing kinase. Upon depletion of glucose in the medium, yeast cells undergo a diauxic shift, accompanied by a carbon metabolic pathway shift, stimulation of mitochondrial function and downregulation of ribosome biogenesis and protein synthesis. We analysed the effect of a pho85Δ mutation on the expression profiles of the genes in this process to investigate whether Pho85 kinase participates in the yeast diauxy. We found that, in the absence of PHO85, a majority of mitochondrial genes were not properly induced, that proteasome‐related and chaperonin genes were more repressed, and that, when glucose was still present in the medium, a certain class of genes involved in ribosome biogenesis (ribosomal protein and rRNA processing genes) was repressed, whereas those involved in gluconeogenesis and the glyoxylate cycle were induced. We also found that PHO85 is required for proper expression of several metal sensor genes and their regulatory genes. These results suggest that Pho85 is required for proper onset of changes in expression profiles of genes responsible for the diauxic shift. Copyright © 2004 John Wiley & Sons, Ltd.