Neurofilament phosphorylation is increased in ventral horn neurons of neonatal rat spinal cord exposed to cerebrospinal fluid from patients with amyotrophic lateral sclerosis
Neurofilament phosphorylation is increased in ventral horn neurons of neonatal rat spinal cord exposed to cerebrospinal fluid from patients with amyotrophic lateral sclerosis
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DOI：
10.1006/neur.1995.0048
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发表时间：
1995-12-01
期刊：
影响因子：
--
通讯作者：
Raju, TR
中科院分区：
文献类型：
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作者：
Rao, MS;Devi, MG;Raju, TR
观察了肌萎缩侧索硬化症(ALS)患者脑脊液(CSF)暴露后，新生大鼠脊髓腹角神经元胞体内神经丝蛋白(NF)的异常磷酸化。将ALS和非ALS神经科患者的脑脊液标本注入3日龄大鼠脊髓蛛网膜下腔。48h后，用SMI-31抗体对脊髓切片进行磷酸化的神经营养因子表位染色。用该抗体对脊髓腹角和背角的神经元胞体进行染色。ALS组大鼠脊髓腹角SMI-31抗体染色的胞体数比非ALS组和未暴露于任何脑脊液组大鼠脊髓腹角的胞体数显著增加3倍。在背角没有观察到神经元胞体染色的增加。神经元胞体的过度磷酸化提示ALS患者脑脊液中的循环因子(S)暴露后，运动神经元(前角)发生了早期的变性改变。(C)1995年学术出版社有限公司。
Aberrant neurofilament (NF) phosphorylation in the soma of the ventral horn neurons of neonatal rat spinal cord is observed following exposure to cerebrospinal fluid (CSF) of patients suffering from Amyotrophic Lateral Sclerosis (ALS). CSF samples from ALS and non-ALS neurological patients were injected into the spinal subarachnoid space of 3 day old rat pups. After 48 h, sections of spinal cords were stained for the presence of phosphorylated NF epitopes with SMI-31 antibody. The number of neuronal soma staining with this antibody in the ventral and dorsal horns sides of the spinal cord was counted. There was a significant 3-fold increase in the number of soma stained with SMI-31 antibody in the ventral horns of rat spinal cords exposed to CSF of patients with ALS compared to cords from rats exposed to CSF of non-ALS patients and those which were not exposed to any CSF samples. Such an increase in staining of neuronal soma was not observed in the dorsal horns. Hyperphosphorylation of neuronal soma suggests an initial stage of degenerative changes occurring in the motor (ventral horn) neurons following exposure to circulating factor(s) in the CSF of patients with ALS. (C) 1995 Academic Press Limited.