Primordial GATA6 macrophages function as extravascular platelets in sterile injury
Primordial GATA6 macrophages function as extravascular platelets in sterile injury
复制标题
DOI：
10.1126/science.abe0595
复制
发表时间：
2021-03-05
期刊：
影响因子：
56.9
通讯作者：
Kubes, P.
中科院分区：
文献类型：
--
作者：
Zindel, J.;Peiseler, M.;Kubes, P.
大多数多细胞生物体都有一个主要的体腔，里面有免疫细胞。在原始物种如紫海胆中，这些细胞执行吞噬功能，但在修复损伤中也至关重要。在哺乳动物中，腹膜腔含有大量的常驻GATA 6（+）巨噬细胞，其功能可能类似。然而，目前尚不清楚悬浮在液相（腹腔液）中的腔巨噬细胞如何识别并向损伤迁移。在这项研究中，我们使用活体显微镜显示，腔巨噬细胞在流体中迅速形成血栓样结构，以响应通过原始清道夫受体富含半胱氨酸的结构域的损伤。腔巨噬细胞聚集体物理密封损伤并促进局灶性病变的快速修复。在医源性手术情况下，这些腔巨噬细胞形成广泛的聚集体，促进称为腹膜粘连的腹内瘢痕组织的生长。
Most multicellular organisms have a major body cavity that harbors immune cells. In primordial species such as purple sea urchins, these cells perform phagocytic functions but are also crucial in repairing injuries. In mammals, the peritoneal cavity contains large numbers of resident GATA6(+) macrophages, which may function similarly. However, it is unclear how cavity macrophages suspended in the fluid phase (peritoneal fluid) identify and migrate toward injuries. In this study, we used intravital microscopy to show that cavity macrophages in fluid rapidly form thrombus-like structures in response to injury by means of primordial scavenger receptor cysteine-rich domains. Aggregates of cavity macrophages physically sealed injuries and promoted rapid repair of focal lesions. In iatrogenic surgical situations, these cavity macrophages formed extensive aggregates that promoted the growth of intra-abdominal scar tissue known as peritoneal adhesions.