The cellulose binding region in Trichoderma reesei cellobiohydrolase I has a higher capacity in improving crystalline cellulose degradation than that of Penicillium oxalicum
The cellulose binding region in Trichoderma reesei cellobiohydrolase I has a higher capacity in improving crystalline cellulose degradation than that of Penicillium oxalicum
复制标题
里氏木霉纤维二糖水解酶 I 中的纤维素结合区域比草酸青霉具有更高的改善结晶纤维素降解的能力
DOI：
10.1016/j.biortech.2018.06.050
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发表时间：
2018-10-01
影响因子：
11.4
通讯作者：
Qu, Yinbo
中科院分区：
文献类型：
--
作者：
Du, Jian;Zhang, Xiu;Qu, Yinbo
用于木质纤维素生物转化的商业纤维素酶制剂主要由真菌里氏木霉（Trichoderma reesei）产生。T.在水解玉米芯渣和微晶纤维素时，reesei纤维素酶混合物比Penicillium pesticum高15%~ 20%。然而，这两种制剂水解超过92%的NaOH丝光Avicel中的纤维素。当添加到Avicel水解残基中时，对P. aceticum纤维素酶的反应性较低，T.在相同的蛋白质负载量下，reesei的纤维素转化率高于其来自P. lauricum和尼日尔的同源蛋白质。进一步的结构域交换实验表明T. reesei CBH I与其结构域间接头和纤维素结合结构域连接。这一结果部分解释了T. reesei纤维素酶对天然结晶纤维素降解的影响，并强调了纤维素结合区在决定纤维素酶水解程度中的重要作用。
Commercial cellulase preparations for lignocellulose bioconversion are mainly produced by the fungus Trichoderma reesei. The maximum cellulose conversion of T. reesei cellulase mixture was 15%-20% higher than that of Penicillium oxalicum in the hydrolysis of corncob residue and Avicel. Nevertheless, both preparations hydrolyzed more than 92% of cellulose in NaOH-mercerized Avicel. When added to Avicel hydrolysis residue that was less reactive to P. oxalicum cellulases, cellobiohydrolase I (CBH I) from T. reesei resulted in a higher cellulose conversion than its homologous proteins from P. oxalicum and Aspergillus niger at the same protein loadings. Further domain exchange experiment attributed the high hydrolytic efficiency of T. reesei CBH I to its inter-domain linker and cellulose-binding domain. The results in part explained the superior performance of T. reesei cellulases on the degradation of native crystalline cellulose, and highlighted the important role of cellulose-binding region in determining the degree of hydrolysis by cellulases.