The proliferative and toxic effects of ultraviolet light and inflammation on epidermal pigment cells.
The proliferative and toxic effects of ultraviolet light and inflammation on epidermal pigment cells.
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紫外线和炎症对表皮色素细胞的增殖和毒性作用。
DOI：
10.1111/1523-1747.ep12493267
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Traynor,FF
中科院分区：
文献类型：
--
作者：
Nordlund,JJ;Ackles,AE;Traynor,FF
小鼠的耳朵可用于研究紫外线对表皮色素细胞的影响。通过用工程卡尺测量耳朵的肿胀，可以很容易地评估穿透皮肤引起炎症反应的光量。耳朵的炎症反应与所传递的光的剂量呈现线性关系。我们观察到，当每天重复一定剂量的短波紫外线时，它是非炎症性的，可诱导中度至重度炎症。小剂量的补骨脂素和长时间暴露于UVA（PUVA）比大量的补骨脂素和短时间暴露于光更容易引起炎症。短波紫外线和PUVA的剂量仅产生皮肤的最小炎症，刺激表皮黑色素细胞的增殖。相比之下，剂量大到足以在皮肤中引起显著炎症反应的PUVA似乎对色素细胞有害并杀死它们或仅引起最小的增殖反应。炎症反应本身似乎并不刺激或抑制黑素细胞的增殖。前列腺素A、E和F2α对表皮色素细胞的增殖无影响。相反，二甲基亚砜（DMSO）和过敏性接触性皮炎增加色素细胞的数密度。类固醇可以阻断酪氨酸酶的功能。我们的实验表明，色素细胞，像许多其他种类的细胞一样，对损伤敏感，至少可以被大剂量的PUVA杀死。
The ear of the mouse is useful for studying the effects of ultraviolet light on epidermal pigment cells. The quantity of light penetrating into the skin causing an inflammatory response can be assessed easily by measuring with an engineering calipers the swelling of the ear. The inflammatory response of the ear exhibits a linear relationship to the dose of light delivered. We observed that doses of shortwave ultraviolet light which are noninflammatory when repeated at daily intervals induce moderate to severe inflammation. Small doses of psoralen and prolonged exposure to UVA (PUVA) were more inflammatory than larger amounts of psoralen and short exposure to light.Doses of shortwave ultraviolet light and PUVA which produce only a minimal inflammation of the skin stimulate the proliferation of epidermal melanocytes. In contrast, PUVA in doses sufficiently large to cause a marked inflammatory reaction in the skin seems injurious to pigment cells and kills them or causes only a minimal proliferative response. The inflammatory reaction itself does not seem to stimulate or inhibit the proliferation of melanocytes. Prostaglandins A, E, and F2αhave no effect on the proliferation of epidermal pigment cells. In contrast, dimethyl sulfoxide (DMSO) and allergic contact dermatitis increase the numerical density of pigment cells. Steroids may block the function of the enzyme tyrosinase. Our experiments indicate that pigment cells, like many other varieties of cells, are susceptible to injury and can be killed at least by large doses of PUVA.