Unexpected elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases
Unexpected elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases
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DOI：
10.1271/bbb.69.1418
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发表时间：
2005-07-01
影响因子：
1.6
通讯作者：
Sambongi, Y
中科院分区：
文献类型：
--
作者：
Kojima, N;Yamanaka, M;Sambongi, Y
缺乏DsbA、DsbB或DsbD(周质中形成二硫键所需的蛋白质)的大肠杆菌突变株不能像以前观察到的细菌那样产生线粒体或叶绿体细胞色素c。然而，出乎意料的是，在没有DSB蛋白的情况下，来自高温菌Aquifex aeolicus的细胞色素C-555(Ac(555))在没有DSB蛋白的情况下产生，是有DSB蛋白的三倍。这些结果表明，DSB蛋白不是在大肠杆菌中生产AA C-555所必需的，可能是因为与其他蛋白相比，DSB蛋白具有极高的嗜热性。
Mutant strains of Escherichia coli lacking DsbA, DsbB, or DsbD (proteins required for disulfide bond formation in the periplasm) did not produce mitochondrial or chloroplast cytochromes c, as previously observed for bacterial ones. Unexpectedly, however, cytochrome C-555 (AA c(555)) from a hyperthermophile, Aquifex aeolicus, was produced in the E. coli periplasm without Dsb proteins, three times more than with them. These results indicate that the Dsb proteins are not necessarily required for AA C-555 production in E. coli, possibly because of hyperthermophilic origin compared with the others.