Detection of adenosine using surface-enhanced Raman scattering based on structure-switching signaling aptamer
Detection of adenosine using surface-enhanced Raman scattering based on structure-switching signaling aptamer
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基于结构转换信号适体的表面增强拉曼散射检测腺苷。
DOI：
10.1016/j.bios.2008.03.013
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发表时间：
2008-09-15
影响因子：
12.6
通讯作者：
Yu, Ru-Qin
中科院分区：
文献类型：
--
作者：
Chen, Ji-Wei;Liu, Xue-Ping;Yu, Ru-Qin
在本研究中，我们报告了一种新的检测腺苷的方法，使用表面增强拉曼散射（Sers）传感平台的基础上的结构转换适体。首先，银包覆Au胶体膜上的抛光的金盘制备作为增强基板和修饰的巯基化捕获DNA。扩增的抗腺苷适体和四甲基罗丹明标记的DNA（表示为TMR-DNA）形成适体/DNA双链体，其中TMR-DNA也可以与捕获DNA完全杂交。因此，腺苷的引入触发适体从适体/DNA双链体到适体/靶复合物的结构转换。结果，释放的TMR-DNA被捕获到Sers基底上，导致Sers信号增加。在优化的测定条件下，达到了宽线性动态范围（2.0 x 10（-8）M至2 x 10（-6）M）和低检测限（1.0 x 10（-8）M）。此外，实现了高选择性、稳定性和容易再生。成功的测试证明了腺苷检测策略的可行性。(C)2008 Elsevier B. V.保留所有权利。
In the present study, we report a novel sensitive method for the detection of adenosine using surface-enhanced Raman scattering (SERS) sensing platform based on a structure-switching aptamer. First, Ag-clad Au colloids film on a polished gold disc is prepared as enhanced substrate and modified with thiolated capture DNA. The formation of an aptamer/DNA duplex of expanded anti-adenosine aptamer and tetramethylrhodamine-labeled DNA (denoted TMR-DNA) is then developed, in which TMR-DNA could also hybridize completely with capture DNA. The introduction of adenosine thus triggers structure switching of the aptamer from aptamer/DNA duplex to aptamer/target complex. As a result, the released TMR-DNA is captured onto the SERS substrate, resulting in an increase of SERS signal. Under optimized assay conditions, a wide linear dynamic range (2.0 x 10(-8) M to 2 x 10(-6) M) was reached with low detection limit (1.0 x 10(-8) M). Moreover, high selectivity, stability and facile regeneration are achieved. The successful test demonstrates the feasibility of the strategy for adenosine assay. (C) 2008 Elsevier B.V. All rights reserved.