Random sampling of evolution time space and Fourier transform processing
Random sampling of evolution time space and Fourier transform processing
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DOI：
10.1007/s10858-006-9077-y
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发表时间：
2006-11-01
影响因子：
2.7
通讯作者：
Zhukov, Igor
中科院分区：
文献类型：
--
作者：
Kazimierczuk, Krzysztof;Zawadzka, Anna;Zhukov, Igor
本文介绍了傅里叶变换在三维核磁共振谱处理中的应用。2D FT是针对成对的频率计算的，而不是传统的一维变换序列。通过仿真和实验研究了不同随机分布的信噪比和线宽。实验实例包括C-13 N-15标记的泛素样品的3D HNCA、HNCACB和N-15限制的NOESY-HSQC谱。实验结果表明，该方法具有普遍的适用性，与同时间记录的常规光谱相比，分辨率有了显著的提高。
Application of Fourier Transform for processing 3D NMR spectra with random sampling of evolution time space is presented. The 2D FT is calculated for pairs of frequencies, instead of conventional sequence of one-dimensional transforms. Signal to noise ratios and linewidths for different random distributions were investigated by simulations and experiments. The experimental examples include 3D HNCA, HNCACB and N-15-limited NOESY-HSQC spectra of C-13 N-15 labeled ubiquitin sample. Obtained results revealed general applicability of proposed method and the significant improvement of resolution in comparison with conventional spectra recorded in the same time.