Electrochemical detection of Salmonella using an invA genosensor on polypyrrole-reduced graphene oxide modified glassy carbon electrode and AuNPs-horseradish peroxidase-streptavidin as nanotag
Electrochemical detection of Salmonella using an invA genosensor on polypyrrole-reduced graphene oxide modified glassy carbon electrode and AuNPs-horseradish peroxidase-streptavidin as nanotag
复制标题
使用聚吡咯还原氧化石墨烯修饰玻碳电极上的 invA 基因传感器和 AuNPs-辣根过氧化物酶-链霉亲和素作为纳米标签对沙门氏菌进行电化学检测
DOI：
10.1016/j.aca.2019.05.012
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发表时间：
2019-10-03
影响因子：
6.2
通讯作者：
Gunasekaran, Sundaram
中科院分区：
文献类型：
--
作者：
Ye, Yongkang;Yan, Wuwen;Gunasekaran, Sundaram
利用invA基因传感器构建了一种快速灵敏的沙门氏菌检测电化学传感器。该生物传感技术基于聚还原氧化石墨烯（py - rgo）纳米复合材料修饰的玻璃碳电极（GCE）和辣根过氧化物酶-亲和素生物功能化金纳米粒子（AuNPs-HRP-SA）的信号放大。将室温原位聚合合成的py -官能化氧化石墨烯（py - go）在60℃下进行化学还原，制备了py -还原氧化石墨烯。以AuNPs-HRP-SA为纳米标签，在对苯二酚（HQ）存在下通过酶还原H2O2扩增检测信号。在最佳条件下，生物传感器输出的差分脉冲电压（DPV）信号与靶基因浓度的对数在1.0 × 10(-16) ~ 1.0 × 10(-10) M范围内呈线性关系，检出限（LOD）为4.7 × 10(-17) M，沙门氏菌的检出范围为9.6 ~ 9.6 × 10(4) CFU mL(-1)， LOD为8.07 CFU mL（-1）。该传感器具有良好的再生能力、良好的选择性、重复性和稳定性，可作为沙门氏菌筛选的替代方法。(C) 2019 Elsevier B.V.版权所有
A rapid and sensitive electrochemical biosensor was constructed to detect Salmonella using invA gene biosensor. The biosensing was based on polyrrole-reduced graphene oxide (PPy-rGO) nanocomposite modified glassy carbon electrode (GCE) and signal amplification with horseradish peroxidase-streptavidin biofunctionalized gold nanoparticles (AuNPs-HRP-SA). PPy-rGO was prepared at 60 degrees C by chemical reduction of PPy-functionalized graphene oxide (PPy-GO) that was synthesized by in situ polymerization at room temperature. The detection signal was amplified via enzymatic reduction of H2O2 in the presence of hydroquinone (HQ) using AuNPs-HRP-SA as nanotag. Under optimal conditions, the differential pulse voltametric (DPV) signal from the biosensor was linearly related to the logarithm of target invA gene concentrations from 1.0 x 10(-16) to 1.0 x 10(-10) M, and the limit of detection (LOD) was 4.7 x 10(-17) M. The biosensor can also detect Salmonella in the range of 9.6 to 9.6 x 10(4) CFU mL(-1), with LOD of 8.07 CFU mL(-1). The biosensor showed good regeneration ability, acceptable selectivity, repeatability and stability, which bode well as an alternative method for Salmonella screening. (C) 2019 Elsevier B.V. All rights reserved.