Anti-inflammatory potential of Lactobacillus reuteri LM1071 via eicosanoid regulation in LPS-stimulated RAW264.7 cells
Anti-inflammatory potential of Lactobacillus reuteri LM1071 via eicosanoid regulation in LPS-stimulated RAW264.7 cells
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DOI：
10.1111/jam.15331
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发表时间：
2021-11-16
影响因子：
4
通讯作者：
Park, Woo Jung
中科院分区：
文献类型：
--
作者：
Jang, A-yeong;Rod-in, Weerawan;Park, Woo Jung
目的：研究罗氏乳杆菌LM1071对内毒素诱导的炎症RAW264.7细胞的抗炎作用。方法与结果：采用脂多糖刺激RAW264.7细胞，评价其抗炎活性。分析IL-1β、IL-6、肿瘤坏死因子-α等8个免疫相关基因的基因表达水平和COX-1、COX-2的蛋白产生水平。结论：本研究表明，罗氏乳杆菌LM1071通过抑制NO、PGE1和PGE2等炎症介质、促炎细胞因子和COX蛋白的产生而具有抗炎作用。还能促进IL-11、BMP4、LEFTY2和EET代谢物等炎症相关基因的产生。研究的意义和影响：罗氏乳杆菌是食品发酵的关键细菌之一。它存在于人和动物的胃肠道系统中。多项研究表明，路氏乳杆菌对寄主健康有重要影响。目前的研究首次证明罗氏乳杆菌对炎症具有良好的作用，其中包括二十烷类化合物(PGE1和PGE2)的变化，这是最重要的生物标志物之一，此外还有二十烷类相关基因和蛋白质(COX-2)的变化。
Aims: To investigate anti-inflammatory effects of Lactobacillus reuteri LM1071 in lipopolysaccharides (LPS)-induced inflammation RAW264.7 cells.Methods and Results: To evaluate anti-inflammatory activities of L. reuteri LM1071, LPS-stimulated RAW264.7 cells were used. Gene expression levels of eight immune-associated genes including IL-1 beta, IL-6 and TNF-alpha and protein production levels of COX-1 and COX-2 were analysed. Moreover, the production of eicosanoids as important biomarkers for anti-inflammation was determined.Conclusions: The current study demonstrates that L. reuteri LM1071 has anti-inflammatory potential by inhibiting the production of inflammation mediators such as NO, eicosanoids such as PGE1 & PGE2, pro-inflammatory cytokines and COX proteins. It can also enhance the production of inflammatory associated genes such as IL-11, BMP4, LEFTY2 and EET metabolite.Significance and Impact of the Study: Lactobacillus reuteri is one of the crucial bacteria for food fermentation. It can be found in the gastrointestinal system of human and animals. Several studies have shown that L. reuteri has valuable effects on host health. The current study firstly demonstrated that L. reuteri has a beneficial effect on the inflammation containing the variation of eicosanoids (PGE1 and PGE2) which are one of the most important biomarkers and moreover eicosanoid-associated genes as well as proteins (COX-2).