Sensitivity of African swine fever virus to type I interferon is linked to genes within multigene families 360 and 505.
Sensitivity of African swine fever virus to type I interferon is linked to genes within multigene families 360 and 505.
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DOI：
10.1016/j.virol.2016.03.019
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发表时间：
2016-06
期刊：
影响因子：
3.7
通讯作者：
Netherton CL
中科院分区：
文献类型：
--
作者：
Golding JP;Goatley L;Goodbourn S;Dixon LK;Taylor G;Netherton CL
非洲猪瘟病毒(ASFV)引起猪的一种致死性出血性疾病。关于干扰素在ASFV感染中的作用，有相互矛盾的报道。因此，我们分析了ASFV与猪干扰素在体内和体外的相互作用。弱毒株Our T88/3缺乏多基因家族(MGF)360和MGF505基因，在感染后第3天即可诱导猪循环中具有生物活性的干扰素，而Our T88/3弱毒株则不能。在体外，用ASFV感染富含树突状细胞的猪白细胞，可诱导高水平的干扰素，提示急性ASF动物可能是干扰素的来源。在干扰素处理的巨噬细胞中，我们的T88/3病毒的复制减少了，但强毒病毒的复制没有减少，一种与我们的T88/3病毒缺乏的基因相似的重组病毒也受到了抑制。这些发现表明，除了抑制干扰素的诱导，MGF360和MGF505基因还使ASFV能够克服抗病毒状态。非洲猪瘟病毒的强毒株在感染期间会诱导干扰素。强毒但未减毒的ASFV株对猪巨噬细胞中干扰素的抗病毒作用具有抵抗力。对干扰素的敏感性与多基因家族360和多基因家族530内的基因有关。树突状细胞是体内检测到的干扰素的潜在来源。
African swine fever virus (ASFV) causes a lethal haemorrhagic disease of pigs. There are conflicting reports on the role of interferon in ASFV infection. We therefore analysed the interaction of ASFV with porcine interferon, in vivo and in vitro. Virulent ASFV induced biologically active IFN in the circulation of pigs from day 3-post infection, whereas low virulent OUR T88/3, which lacks genes from multigene family (MGF) 360 and MGF505, did not. Infection of porcine leucocytes enriched for dendritic cells, with ASFV, in vitro, induced high levels of interferon, suggesting a potential source of interferon in animals undergoing acute ASF. Replication of OUR T88/3, but not virulent viruses, was reduced in interferon pretreated macrophages and a recombinant virus lacking similar genes to those absent in OUR T88/3 was also inhibited. These findings suggest that as well as inhibiting the induction of interferon, MGF360 and MGF505 genes also enable ASFV to overcome the antiviral state. Virulent strains of African swine fever virus induces interferon during infection. Virulent, but not attenuated strains of ASFV are resistant to the antiviral effects of interferon in porcine macrophages. Sensitivity to interferon is linked to genes within multigene family 360 and multigene family 530. Dendritic cells are a potential source of the interferon detected in vivo.