OmpA is a virulence factor of Riemerella anatipestifer
OmpA is a virulence factor of Riemerella anatipestifer
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OmpA是鸭疫里默氏菌的毒力因子
DOI：
10.1016/j.vetmic.2011.01.022
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发表时间：
2011-06-02
影响因子：
3.3
通讯作者：
Yu, Shengqing
中科院分区：
文献类型：
--
作者：
Hu, Qinghai;Han, Xiangan;Yu, Shengqing
鸭疫里默氏菌感染可能是世界范围内最重要的经济疾病。鸭疫R.引起鸭败血症，但对其发病机制的分子基础和所涉及的毒力因素了解甚少。本研究通过从疫鼠R. 2血清型菌株Th4中删除ompA基因，构建突变株Th4 Delta ompA，探讨疫鼠R. ompA是否是一个重要的毒力因子。结果表明，虽然Th4 δ ompA在胰蛋白酶豆汤（TSB）或TSB琼脂上的生长曲线、细菌和菌落形态与其亲本菌株Th4相似，但突变菌株对Vero细胞的粘附和侵袭能力显著降低。测定两株菌株对10日龄樱桃谷雏鸭的中位致死量（LD(50)）。结果表明，Th4 δ ompA突变体的LD（50）为bbbb10（10）个菌落形成单位（CFU），与Th4突变体的LD（50）为4.41 × 10(8)个菌落形成单位（CFU）相比显著降低。进一步的分析表明，感染了Th4 δ ompA突变体的雏鸭的血液细菌负荷远低于感染了Th4突变体的雏鸭。结果表明，OmpA是禽疫鼠的毒力因子，可能具有粘附素的作用。(C) 2011 Elsevier B.V.版权所有
Riemerella anatipestifer infection is probably the most economically important disease of farm ducks worldwide. The pathogen R. anatipestifer causes septicemia anserum exsudativa in ducks, but little is known about the molecular basis of its pathogenesis and the virulence factors involved. In this study, by deleting ompA gene from R. anatipestifer serotype 2 strain Th4, we constructed a mutant strain Th4 Delta ompA to investigate whether R. anatipestifer OmpA is an important virulence factor. Results showed that although the growth curve, bacterial and colony morphology of Th4 Delta ompA in tryptic soybean broth (TSB) or on TSB agar were similar to its parent strain Th4, the adhesion and invasion capacities of mutant strain to Vero cells were decreased significantly. Furthermore, the median lethal dose (LD(50)) of both strains was determined to measure the virulence with 10-day-old Cherry Valley ducklings. The results showed that LD(50) of Th4 Delta ompA mutant was > 10(10) colony forming units (CFU), it was attenuated significantly in comparison with that of Th4 which LD(50) was 4.41 x 10(8) CFU. Additional analysis indicated that blood bacterial loading of ducklings infected with the Th4 Delta ompA mutant were much lower than those of Th4-infected ducklings. The results demonstrate that OmpA is a virulence factor of R. anatipestifer, and that it may act as an adhesin. (C) 2011 Elsevier B.V. All rights reserved.