Distribution of dopamine receptors D1- and D2-immunoreactive neurons in the dorsal motor nucleus of vagus in rats
Distribution of dopamine receptors D1- and D2-immunoreactive neurons in the dorsal motor nucleus of vagus in rats
复制标题
大鼠迷走神经背运动核多巴胺受体 D1 和 D2 免疫反应神经元的分布
DOI：
10.1016/j.autneu.2013.01.007
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发表时间：
2013-06-01
影响因子：
2.7
通讯作者：
Zhu, Jin-Xia
中科院分区：
文献类型：
--
作者：
Cai, Qing-Qing;Zheng, Li-Fei;Zhu, Jin-Xia
迷走神经背侧运动核（DMV）在胃肠功能调节中起重要作用。多巴胺（DA）通过多巴胺受体（DRs）对DMV运动神经元发挥重要的神经调节作用。然而，DR及其神经化学表型在DMV中的分布尚不清楚。本研究采用免疫荧光双标记技术结合共聚焦显微镜观察了大鼠DMV内DR D-1和D-2免疫反应（IR）神经元的分布及其神经化学表型。结果表明，D-1和D-2在整个DMV中有相当数量的表达。DMV内可见大量胆碱乙酰转移酶（ChAT）阳性神经元和少量酪氨酸羟化酶（TH）阳性神经元。结果表明，D-1和D-2在大鼠DMV胆碱能和儿茶酚胺能神经元中广泛分布。DR可能在DA调节DMV胆碱能和儿茶酚胺能神经元活动中起重要作用。(C)2013爱思唯尔有限公司版权所有。
The dorsal motor nucleus of vagus (DMV) plays an important role in the regulation of gastrointestinal function. Dopamine (DA) exerts potent neuromodulatory effects on the motoneurons in the DMV via dopamine receptors (DRs). However, the distribution of DRs and their neurochemical phenotypes in the DMV are unclear. In the present study, the distribution of DRs D-1- and D-2-immunoreactive (IR) neurons and their neurochemical phenotypes in the DMV of rats were investigated using a double-labeling immunofluorescence technique combined with confocal microscopy. The results indicated that a considerable quantity of D-1 and D-2 was expressed throughout the DMV. A large amount of choline acetyltransferase (ChAT)-IR and a few tyrosine hydroxylase (TH)-IR neurons were observed in the DMV. Nearly all of the neurons were also D-1-IR and D-2-IR. In conclusion, the present study demonstrates the wide distribution of D-1 and D-2 in the cholinergic and catecholaminergic neurons in the DMV of rats. The DRs might play an important role in the regulation of DA on the activity of cholinergic and catecholaminergic neurons in the DMV. (C) 2013 Elsevier B.V. All rights reserved.