Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells
Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells
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DOI：
10.1016/j.biomaterials.2009.09.041
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发表时间：
2010-01-01
期刊：
影响因子：
14
通讯作者：
Hollister, Scott J.
中科院分区：
文献类型：
--
作者：
Kemppainen, Jessica M.;Hollister, Scott J.
人们普遍认为支架的渗透性对软骨形成有重要影响。然而，由于渗透性在以前的研究中没有严格控制，因此关于渗透性如何影响原代软骨细胞或祖细胞的软骨再生没有明确的结论。在这里，我们使用精确设计的聚（E-己内酯）（PCL）支架，探讨了支架渗透性对软骨细胞和骨髓基质细胞（BMSC）的基质产生和细胞分化的体外影响，其中高渗透性设计的渗透性是最低渗透性设计的5.25倍。我们发现支架的渗透性以相反的方式影响软骨细胞和骨髓基质细胞的成软骨性能。降低的支架渗透性导致软骨细胞产生软骨基质的增加，促进聚集蛋白聚糖含量和胶原蛋白2：胶原蛋白1基因表达比率的增加。另一方面，在该模型中，增加的支架渗透性更有利于BMSC向软骨形成谱系分化。通过物理设计参数（如渗透性）指导细胞分化和软骨形成的能力建立了将软骨形成潜力纳入材料设计的能力。(C)2009爱思唯尔有限公司保留所有权利。
It has been widely postulated that scaffold permeability has a significant influence on chondrogenesis. However, since permeability has not been rigorously controlled in previous studies, there is no definitive conclusion as to how permeability affects cartilage regeneration by primary chondrocytes or progenitor cells. Here we explored the in vitro effects of scaffold permeability on matrix production and cellular differentiation of chondrocytes and bone marrow stromal cells (BMSCs) using precisely designed poly(E-caprolactone) (PCL) scaffolds in which the high permeability design was 5.25 times more permeable than the lowest permeability design. We found that scaffold permeability affects the chondrogenic performance of chondrocytes and bone marrow stromal cells in opposite ways. Decreased scaffold permeability results in an increase in cartilaginous matrix production by chondrocytes, promoting increases in aggrecan content and collagen 2: collagen 1 gene expression ratios. On the other hand, increased scaffold permeability is more favorable for differentiation of BMSCs down a chondrogenic lineage in this model. The ability to direct cell differentiation and chondrogenesis through a physical design parameter, such as permeability, establishes the capability to incorporate chondrogenic potential into a material design. (C) 2009 Elsevier Ltd. All rights reserved.