Crk is required for apoptosis in Xenopus egg extracts
Crk is required for apoptosis in Xenopus egg extracts
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DOI：
10.1093/emboj/16.2.230
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发表时间：
1997-01-15
期刊：
影响因子：
11.4
通讯作者：
Kornbluth, S
中科院分区：
文献类型：
--
作者：
Evans, EK;Lu, W;Kornbluth, S
细胞凋亡对于多细胞生物的发育和稳态是必不可少的。最近，从非洲爪蟾卵制备的无细胞提取物显示出在体外重现细胞内细胞凋亡途径。虽然许多刺激已经显示出在多种细胞类型中触发细胞凋亡，但是涉及细胞凋亡的细胞内信号传导途径在很大程度上仍然未知。在这里，我们表明，除了含有从衔接蛋白crk的磷酸酪氨酸结合（SH2）结构域的重组蛋白，但不是那些来自其他信号蛋白的面板，调用防止非洲爪蟾卵提取物系统中的细胞凋亡。此外，从鸡蛋提取物中免疫去除内源性crk蛋白，或向这些提取物中加入抗crk抗血清，可以防止细胞凋亡。通过加入重组crk蛋白，这些提取物可以恢复细胞凋亡的能力。这些结果直接表明crk参与了凋亡信号传导。
Apoptosis is essential for the development and homeostasis of multicellular organisms, Recently, a cell-free extract prepared from Xenopus eggs was shown to recapitulate intracellular apoptotic pathways in vitro, While many stimuli have been shown to trigger apoptosis in a variety of cell types, the intracellular signaling pathways involved in apoptosis remain largely unknown, Here we show that addition of a recombinant protein containing the phosphotyrosine binding (SH2) domain from the adaptor protein crk, but not those derived from a panel of other signaling proteins, call prevent apoptosis in the Xenopus egg extract system. Furthermore, immunodepletion of endogenous crk protein from the egg extracts, or addition of anti-crk antisera to these extracts, prevents apoptosis. The ability to undergo apoptosis can be restored to these extracts by addition of recombinant crk protein. These results directly demonstrate that crk participates in apoptotic signaling.