Interfacing Cell-Based Assays in Environmental Scanning Electron Microscopy Using Dielectrophoresis
Interfacing Cell-Based Assays in Environmental Scanning Electron Microscopy Using Dielectrophoresis
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DOI：
10.1021/ac2002142
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发表时间：
2011-04-15
影响因子：
7.4
通讯作者：
Wlodkowic, Donald
中科院分区：
文献类型：
--
作者：
Khoshmanesh, Khashayar;Akagi, Jin;Wlodkowic, Donald
介绍了介电泳法(DEP)活细胞捕获技术的发展及其与环境扫描电子显微镜(ESEM)的接口。利用光刻和剥离技术在玻璃衬底上制备了DEP微电极阵列。将芯片阵列应用于DEP捕获的人白血病细胞的ESEM分析。这项工作提供了DEP细胞保留和捕获技术与环境扫描电子显微镜的概念验证接口，以提供对单个未黏附细胞的高分辨率分析。
Development of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work, provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells.