A non-destructive technique for 3-D microstructural phenotypic characterisation of bones in genetically altered mice:: preliminary data in growth hormone transgenic animals and normal controls
A non-destructive technique for 3-D microstructural phenotypic characterisation of bones in genetically altered mice:: preliminary data in growth hormone transgenic animals and normal controls
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DOI：
10.1007/s004290050225
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发表时间：
1999-03-01
期刊：
影响因子：
--
通讯作者：
Eckstein, F
中科院分区：
文献类型：
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作者：
Graichen, H;Lochmüller, EM;Eckstein, F
本文介绍了一种非破坏性的三维技术，用于遗传改变小鼠骨骼元素的显微结构表型表征。显示了牛生长激素转基因动物和对照同窝仔的初步数据。该技术是基于微计算机断层扫描（μ CT）和数字后处理，并允许在轴向和外周骨骼的皮质骨和骨小梁室的差分定量分析。对6只动物的股骨远端和第一腰椎椎体进行轴向CT扫描，各向同性分辨率为20 μ m。骨膜表面和骨髓腔被完全自动分割，骨小梁和皮质隔室被交互分离。三维重建后，选择不同的感兴趣区域（骨干，干骺端和骨骺）进行分析。转基因动物的股骨和椎骨在大小、形状和骨小梁排列上与对照动物相比表现出明显的差异。总骨量增加了2 - 3倍，但骨小梁比皮质骨增加得多（高达12倍）。转基因动物表现出骨小梁与皮质骨的比例增加（股骨干为0.90 - 1.27 vs 0.14 - 0.36）和骨小梁体积分数升高（股骨干骺端为49%-73% vs 18%-43%）。正常动物和转基因动物的平均三维皮质厚度相似（骨干和干骺端的值在93 μ m和232 μ m之间），但转基因动物的最小皮质厚度较低（骨干为22 μ m至31 μ m vs 54 μ m至110 μ m）。所提出的技术适用于遗传改变小鼠骨结构的表型表征。
A non-destructive, three-dimensional technique for microstructural phenotypic characterisation of skeletal elements in genetically altered mice is presented. Preliminary data in bovine growth-hormone transgenic animals and control littermates are shown. The technique is based on microcomputed tomography (mu CT) and digital postprocessing and allows for a differential quantitative analysis of the cortical and trabecular bone compartments in the axial and peripheral skeleton. The distal femora and the first lumbar vertebral bodies of six animals were CT scanned in the axial plane with an isotropic resolution of 20 mu m. The periostal surface and the marrow spaces were segmented fully automatically, and the trabecular and cortical compartments were separated interactively. After 3-D reconstruction, various regions of interest (diaphyseal, metaphyseal and epiphyseal) were selected for the analysis. The femora and vertebrae of the transgenic animals showed obvious differences in size, shape, and trabecular arrangement compared with the control animals. The total bone mass was increased by a factor of two to three, but the trabecular bone was increased much more (up to 12 times) than the cortical bone. The transgenic animals showed an increased ratio of trabecular vs cortical bone (0.90 to 1.27 vs 0.14 to 0.36 in the femoral diaphysis) and an elevated trabecular bone volume fraction (49% to 73% vs 18% to 43% in the femoral metaphysis). The mean 3-D cortical thickness was similar in the normal and transgenic animals (values between 93 mu m and 232 mu m in the dia- and metaphyses), but the minimal cortical thickness was lower in the transgenic animals (22 to 31 mu m vs 54 mu m to 110 mu m in the diaphysis). The technique presented is suitable for phenotypic characterisation of bone structure in genetically altered mice.