Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
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DOI：
10.1016/s0896-6273(00)80701-1
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发表时间：
1999-03-01
期刊：
影响因子：
16.2
通讯作者：
Luo, LQ
中科院分区：
文献类型：
--
作者：
Lee, T;Luo, LQ
我们描述了果蝇中的遗传嵌合系统，其中细胞标记的显性阻遏物被放置在感兴趣的突变基因的反式中。同源染色体之间的有丝分裂重组事件产生纯合突变细胞，这些细胞由于阻遏蛋白的缺失而被专门标记。使用该系统，我们能够使用膜靶向 GFP 标记来可视化大型神经母细胞克隆和单个神经元克隆中的轴突投影和树突精细化。这种新方法可以研究复杂中枢神经系统中神经母细胞增殖、轴突引导和树突精细化的基因功能。作为一个例子，我们证明蘑菇体神经元需要短暂停止基因来延伸和引导其轴突。
We describe a genetic mosaic system in Drosophila, in which a dominant repressor of a cell marker is placed in trans to a mutant gene of interest. Mitotic recombination events between homologous chromosomes generate homozygous mutant cells, which are exclusively labeled due to loss of the repressor. Using this system, we are able to visualize axonal projections and dendritic elaboration in large neuroblast clones and single neuron clones with a membrane-targeted GFP marker. This new method allows for the study of gene functions in neuroblast proliferation, axon guidance, and dendritic elaboration in the complex central nervous system. As an example, we show that the short stop gene is required in mushroom body neurons for the extension and guidance of their axons.