Phosphonic acid monolayers for binding of bioactive molecules to titanium surfaces
Phosphonic acid monolayers for binding of bioactive molecules to titanium surfaces
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DOI：
10.1021/la060754c
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发表时间：
2006-09-12
期刊：
影响因子：
3.9
通讯作者：
Menzel, Henning
中科院分区：
文献类型：
--
作者：
Adden, Nina;Gamble, Lara J.;Menzel, Henning
制备了两种不同的膦酸单层膜，用于将生物活性分子（例如蛋白质 BMP-2）固定在钛表面上。 (11-羟基十一烷基)膦酸和(12-羧基十二烷基)膦酸分子的单层通过简单的浸渍工艺（T-BAG方法）生产。这些单层上的末端官能团被激活（羟基为羰基二咪唑，羧基为 N-羟基琥珀酰亚胺）以结合含胺分子。使用盐酸三氟乙胺和 BMP-2 研究表面的反应性。表面改性过程的每个步骤均通过 X 射线光电子能谱和飞行时间二次离子质谱进行表征。
Two different phosphonic acid monolayer films for immobilization of bioactive molecules such as the protein BMP-2 on titanium surfaces have been prepared. Monolayers of (11-hydroxyundecyl) phosphonic acid and (12-carboxydodecyl) phosphonic acid molecules were produced by a simple dipping process (the T-BAG method). The terminal functional groups on these monolayers were activated (carbonyldiimidazole for hydroxyl groups and N-hydroxysuccinimide for carboxyl groups) to bind amine-containing molecules. The reactivity of the surfaces was investigated using trifluoroethylamine hydrochloride and BMP-2. Each step of the surface modification procedure was characterized by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry.