INTRASTRIATAL GRAFTING OF DOPAMINE-CONTAINING NEURONAL CELL-SUSPENSIONS - EFFECTS OF MIXING WITH TARGET OR NONTARGET CELLS
INTRASTRIATAL GRAFTING OF DOPAMINE-CONTAINING NEURONAL CELL-SUSPENSIONS - EFFECTS OF MIXING WITH TARGET OR NONTARGET CELLS
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DOI：
10.1016/0165-3806(86)90174-4
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
BJORKLUND, A
中科院分区：
文献类型：
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作者：
BRUNDIN, P;ISACSON, O;BJORKLUND, A
在单侧 6-羟基多巴胺诱导的黑质纹状体多巴胺通路损伤的大鼠中，研究了靶细胞和非靶细胞对中脑多巴胺神经元纹状体内移植物生长和功能的影响。来自 14-15 日龄大鼠胎儿（富含发育中的多巴胺神经元）的腹侧中脑细胞悬浮液可以单独移植，也可以与从纹状体（主要多巴胺目标区域）或脊髓（中脑多巴胺神经元的非目标区域）获得的等量细胞混合后移植。与单独的中脑细胞移植物或与脊髓细胞混合的移植物或中脑细胞相比，组合的中脑和纹状体移植物在宿主尾壳核中产生更大的密集神经支配区域。不同类型的移植物中存活的含儿茶酚胺神经元的数量没有显着差异。此外，由强荧光的含儿茶酚胺纤维的小圆岛组成的组合中脑纹状体移植物的生长模式发生了改变，通常与移植的多巴胺神经元密切相关。在随后的生化研究中发现，联合中脑-纹状体移植物表现出的多巴胺水平和周转率与仅包含中脑细胞的移植物没有区别。与其他移植物组相比，中脑-纹状体联合移植物表现出行为效应增强的趋势，即安非他明引起的旋转不对称性更大程度地减少。这表明添加胚胎纹状体靶细胞可以对纹状体移植后体内胎儿多巴胺细胞的形态发育和可能的功能参数产生刺激作用。然而，该结果在原则上与类似体外研究中获得的结果不同，可能是由于本实验中所有组中都存在成体宿主纹状体靶细胞。
The effects of target and non-target cells on the growth and function of intrastriatal grafts of mesencephalic dopamine neurons have been studied in rats with unilateral 6-hydroxydopamine-induced lesions of the nigrostriatal dopamine pathway. Cell suspensions of ventral mesenchephalon from 14-15-day-old rats fetuses (rich in developing dopamine neurons) were either grafted alone or grafted after mixing with equivalent numbers of cells obtained from the striatum (a major dopamine target area) or spinal cord (a non-target area for the mesencephalic dopamine neurons). The combined mesencephalic and striatal grafts gave rise to a greater area of dense innervation in the host caudate-putamen than grafts of mesencephalic cells alone or grafts or mesencephalic cells mixed with spinal cord cells. The number of surviving catecholamine-contaning neurons did not differ significantly in the different types of grafts. In addition, there was an altered outgrowth pattern in the combined mesencephalic-striatal grafts consisting of small round islands of intensively fluorescent catecholamine-containing fibres, often in close association with the grafted dopamine neurons. In a subsequent biochemical study it was found that combined mesencephalic-striatal grafts exhibited dopamine levels and turnover that did not differ from grafts containing mesencephalic cells only. The mesencephalic-striatal cografts showed a trend toward enhanced behavioral effects, in terms of greater reduction in amphetamine-induced rotation asymmetry, when compared to other graft groups. It is suggested that the addition of embryonic striatal target cells can exert stimulatory effects on morphological development, and possibly functional parameters, of fetal dopamine cells also in vivo after intrastriatal grafting. However, the result differ in principle from those obtained in analogous in vitro studies, possibly due to the presence of adult host striatal target cells in all the groups in the present experiments.