Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain
Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain
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DOI：
10.1111/j.1365-2583.2004.00524.x
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Kubo, T
中科院分区：
文献类型：
--
作者：
Paul, RK;Takeuchi, H;Kubo, T
为了促进蜜蜂（Apis mellifera L.）激素控制的研究，分离出蜕皮激素调节基因E74（AmE74）的蜜蜂同源物的cDNA，并分析其表达。北方印迹分析表明，该基因在蜂后腹部有较强表达，在雄蜂和工蜂中无明显表达。原位杂交结果表明，该基因选择性地在卵巢和胃肠道中表达。此外，该基因也选择性地表达在成年工蜂脑中的蘑菇体神经元亚群中。这些发现表明AmE74参与成年蜜蜂的神经功能以及繁殖。
To facilitate studies of hormonal control in the honeybee (Apis mellifera L.), a cDNA for a honeybee homologue of the ecdysteroid-regulated gene E74 (AmE74) was isolated and its expression was analysed. Northern blot analysis indicated strong expression in the adult queen abdomen, and no significant expression in the adult drone and worker abdomens. In situ hybridization demonstrated that this gene was expressed selectively in the ovary and gut in the queen abdomen. Furthermore, this gene was also expressed selectively in subsets of mushroom body interneurones in the brain of the adult worker bees. These findings suggest that AmE74 is involved in neural function as well as in reproduction in adult honeybees.