Cell division -: A histone-H3-like protein in C-elegans
Cell division -: A histone-H3-like protein in C-elegans
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DOI：
10.1038/44062
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发表时间：
1999-10-07
期刊：
影响因子：
64.8
通讯作者：
Henikoff, S
中科院分区：
文献类型：
--
作者：
Buchwitz, BJ;Ahmad, K;Henikoff, S
有丝分裂期间染色体的分离是由染色体的着丝粒区域调节的，着丝粒组织着丝粒，纺锤体微管附着在着丝粒上。许多生物具有单着丝粒染色体，其中着丝粒映射到单个位置，而其他生物，包括线虫，具有全着丝粒染色体，其中非定位的动粒沿着每条染色体的长度延伸。单着丝粒染色体的着丝粒使用含有组蛋白-H3样蛋白的特殊核小体(在哺乳动物中称为CENP-A，在酿酒酵母中称为Cse4)。在这里，我们展示了AC。Eleganshistone-H3-like蛋白是有丝分裂过程中染色体正确分离所必需的，并鉴定了这些全着丝粒染色体的着丝粒，表明全着丝粒和单着丝粒染色体都使用着丝粒的组蛋白-H3-like蛋白。
The segregation of a chromosome during mitosis is mediated by a region of the chromosome known as the centromere, which organizes the kinetochore, to which the spindle microtubules attach. Many organisms have monocentric chromosomes, in which the centromeres map to single loci, whereas others, including the nematodeCaenorhabditis elegans, have holocentric chromosomes, in which non-localized kinetochores extend along the length of each chromosome,. The centromeres of monocentric chromosomes use specialized nucleosomes containing histone-H3-like proteins (known as CENP-A in mammals,,,and Cse4 in the yeastSaccharomyces cerevisiae,). Here we show that aC. eleganshistone-H3-like protein is necessary for the proper segregation of chromosomes during mitosis and identifies the centromeres of these holocentric chromosomes, indicating that both holocentric and monocentric chromosomes use centromeric histone-H3-like proteins.