Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners.
Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners.
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DOI：
10.1038/srep42100
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发表时间：
2017-02-09
影响因子：
4.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Lin Z;Chen M;Dong X;Zheng X;Huang H;Xu X;Chen J
在中国南海，北部湾沿海富营养化使珊瑚礁受到慢性生理压力，严重威胁到珊瑚礁生境。要确定如何珊瑚holobiont可能容忍这样的条件下，我们研究了转录组的健康殖民地的星系珊瑚Galaxea fascicularis和内共生体共生藻从两个珊瑚礁网站经历原始或富营养化的营养制度。我们确定了236和205个基因的差异表达在富营养化主机和共生体，分别。这两个基因集都包括与应激反应和代谢相互作用相关的途径。来自每个合作伙伴的基因的分析揭示了惊人的代谢整合方面的维生素，辅因子，氨基酸，脂肪酸和次级代谢产物的生物合成。这些基因的表达水平支持了在这种珊瑚-藻类共生关系中存在着一种连续的互利共生关系。此外，大量的转录因子、细胞信号转导分子、生物矿化组分和半乳糖苷相关蛋白在G. fascicularis相对于其他珊瑚物种。
In the South China Sea, coastal eutrophication in the Beibu Gulf has seriously threatened reef habitats by subjecting corals to chronic physiological stress. To determine how coral holobionts may tolerate such conditions, we examined the transcriptomes of healthy colonies of the galaxy coral Galaxea fascicularis and its endosymbiont Symbiodinium from two reef sites experiencing pristine or eutrophied nutrient regimes. We identified 236 and 205 genes that were differentially expressed in eutrophied hosts and symbionts, respectively. Both gene sets included pathways related to stress responses and metabolic interactions. An analysis of genes originating from each partner revealed striking metabolic integration with respect to vitamins, cofactors, amino acids, fatty acids, and secondary metabolite biosynthesis. The expression levels of these genes supported the existence of a continuum of mutualism in this coral-algal symbiosis. Additionally, large sets of transcription factors, cell signal transduction molecules, biomineralization components, and galaxin-related proteins were expanded in G. fascicularis relative to other coral species.