A fast, high-affinity fluorescent serotonin biosensor engineered from a tick lipocalin.
A fast, high-affinity fluorescent serotonin biosensor engineered from a tick lipocalin.
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一种由蜱脂质运载蛋白设计而成的快速、高亲和力荧光血清素生物传感器。
DOI：
10.1038/s41592-021-01078-7
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发表时间：
2021
期刊：
影响因子：
48
通讯作者：
Ai,Hui-Wang
中科院分区：
文献类型：
--
作者：
Zhang,Shen;Li,Xinyu;Zhao,Shengyu;Drobizhev,Mikhail;Ai,Hui-Wang
5-羟色胺（5-HT）是一种重要的信号单胺和神经递质。我们报告了一个绿色荧光，基因编码的5-羟色胺传感器（G-GESS）的结构指导工程从5-HT结合脂质运载蛋白在软tickArgas monolakensis。G-GESS具有快速的响应动力学和高亲和力、特异性、亮度和光稳定性。我们使用G-GESS成像5-HT在培养细胞，脑切片和行为小鼠的动力学。
Serotonin (5-HT) is an important signaling monoamine and neurotransmitter. We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tickArgas monolakensis. G-GESS shows fast response kinetics and high affinity, specificity, brightness and photostability. We used G-GESS to image 5-HT dynamics in cultured cells, brain slices and behaving mice.