Intercellular communication through gap junctions: a potential role in pharmacomechanical coupling and syncytial tissue contraction in vascular smooth muscle isolated from the human corpus cavernosum.
Intercellular communication through gap junctions: a potential role in pharmacomechanical coupling and syncytial tissue contraction in vascular smooth muscle isolated from the human corpus cavernosum.
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DOI：
10.1016/0024-3205(91)90489-x
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发表时间：
1991
期刊：
影响因子：
6.1
通讯作者：
George J. Christ;Alonso P. Moreno;Melanie E. Parker;C. Gondre;Mira Valcic;Arnold Melman;D. C. Spray
中科院分区：
文献类型：
--
作者：
George J. Christ;Alonso P. Moreno;Melanie E. Parker;C. Gondre;Mira Valcic;Arnold Melman;D. C. Spray
采用动力学和稳态方案研究了用庚醇阻断细胞间通讯对人阴茎海绵体血管平滑肌α1肾上腺素能受体激活引起的收缩反应的影响。对于稳态研究，19例患者的身体组织条次最大程度地预收缩苯肾上腺素（PE），然后放松的累积添加庚醇。庚醇以浓度依赖性方式完全和可逆地松弛研究的所有组织。然后将庚醇浓度响应数据计算机拟合至一般逻辑方程，以获得PEC 50（消除最大效应一半的浓度的负对数）和斜率因子值，Emax（最大松弛）设定为100%。平均pEC50和斜率因子值分别为2.86±0.04和1.86±0.17。此外，对患者群体（11例）的一个亚组的身体组织的动力学研究表明，用2 mM庚醇预孵育组织导致研究的所有组织中PE诱导收缩的速率和幅度显著降低，而不影响收缩开始的速率常数（kobs）。完整组织的实验结果扩展了我们以前在体外培养的体细胞上的观察，支持了通过缝隙连接进行的细胞间通讯在人血管平滑肌α1肾上腺素能收缩的启动、维持和调节中起重要作用的假设。
Kinetic and steady-state protocols were used to examine the effects of disruption of intercellular communication with heptanol, on contractile responses elicited by activation of the α1-adrenergic receptor in human corporal vascular smooth muscle. For the steady-state studies, strips of corporal tissue from 19 patients were submaximally precontracted with phenylephrine (PE) and then relaxed by the cumulative addition of heptanol. Heptanol completely and reversibly relaxed all tissues studied in a concentration-dependent manner. The heptanol concentration response data were then computer fit to the general logistic equation to obtain pEC50(negative logarithm of the concentration that elicits one-half of the maximal effect) and slope factor values, with Emax(maximal relaxation) set to 100%. The mean pEC50and slope factor values, respectively, were 2.86±0.04 and 1.86±0.17. Furthermore, kinetic studies on corporal tissues from asubsetof the patient population (11 patients) revealed that preincubation of tissues with 2 mM heptanol caused a significant decrease in both the rate and magnitude of PE-induced contractions in all tissues studied, without affecting the rate constant for onset of contraction (kobs). The present results on intact tissue extend our previous observations on cultured corporal cells, and support the supposition that intercellular communication through gap junctions may play an important role in the initiation, maintenance and modulation of α1-adrenergic contractions in human vascular smooth muscle.