Characterization of a laccase gene from the white-rot fungi Trametes sp 5930 isolated from Shennongjia Nature Reserve in China and studying on the capability of decolorization of different synthetic dyes
Characterization of a laccase gene from the white-rot fungi Trametes sp 5930 isolated from Shennongjia Nature Reserve in China and studying on the capability of decolorization of different synthetic dyes
复制标题
白腐真菌栓菌漆酶基因的表征。
DOI：
10.1016/j.bej.2011.07.006
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发表时间：
2011-11-15
影响因子：
3.9
通讯作者：
Jiang, Mulan
中科院分区：
文献类型：
--
作者：
Yang, Yang;Ma, Fuying;Jiang, Mulan
漆酶属于多铜氧化酶家族，其在生物技术和工业应用中特别有用。从神农架自然保护区分离到一株产漆酶的白腐真菌Trametessp.5930。Trametessp.5930漆酶产量高，对染料有较好的脱色效果。漆酶在该菌对不同染料的脱色过程中起着非常重要的作用。然后从栓菌（Trametes sp.）5930中克隆了漆酶基因lac 5930 -1及其相应的全长cDNA。lac 5930 -1的全长cDNA为1563 bp，编码一个由499个氨基酸组成的成熟漆酶蛋白，前加一个21个氨基酸的信号肽。在毕赤酵母中成功表达了lac 5930 -1基因，通过基因表达验证了lac 5930 -1编码活性漆酶的功能。利用高效表达lac 5930 -1的酵母菌产生的重组漆酶，具有对不同染料的脱色能力。漆酶对不同染料的脱色能力与其活性呈正相关，为漆酶在工业染料脱色中的重要作用提供了有力的证据。(C)出版社：Elsevier B. V.
Laccase belongs to a family of multi-copper oxidases which is especially useful for biotechnological and industrial applications. A laccase-producing white-rot fungi strain designated as Trametes sp. 5930 was nearly isolated from Shennongjia Nature Reserve in China. Trametes sp. 5930 had the high yield of laccase and was capable of clecolorizing different dyes efficiently. Laccase played a very important role in the decolorization of different dyes by this fungus. The laccase gene lac5930-1 and its corresponding full-length cDNA were then cloned and characterized from Trametes sp. 5930. The 1563 bp full-length cDNA of lac5930-1 encoded a mature laccase protein consisting of 499 amino acids preceded by a signal peptide of 21 amino acids. lac5930-1 gene was successfully expressed in Pichia pastoris, which verified the function of lac5930-1 encoding active laccase by means of gene expression. The recombinant laccase produced by the yeast transformant in which lac5930-1 was efficiently expressed, conferred the ability to decolorize different dyes. The capability of decolorizing different dyes was positively related to the laccase activity, which provided strong evidence for the important function of laccase used in decolorizing industrial dyes. (C) 2011 Published by Elsevier B.V.