Gastric cancer increase the percentage of intermediate (CD14++CD16+) and nonclassical (CD14+CD16+) monocytes
Gastric cancer increase the percentage of intermediate (CD14++CD16+) and nonclassical (CD14+CD16+) monocytes
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DOI：
10.5114/ceji.2012.32725
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发表时间：
2012-01-01
影响因子：
1.3
通讯作者：
Zegarski, Wojciech
中科院分区：
文献类型：
--
作者：
Eljaszewicz, Andrzej;Jankowski, Michal;Zegarski, Wojciech
前言：胃癌是世界范围内最常见的恶性肿瘤之一。尽管有治疗选择，预后是不利的，特别是当处理晚期癌症时。与其他恶性肿瘤相似，胃癌的发生与巨噬细胞浸润增加有关。单核细胞是组织巨噬细胞的外周血前体。根据CD 14和CD 16受体表达的关系，人外周血单核细胞可分为：“经典单核细胞”CD 14 ++ CD 16-、中间型单核细胞CD 14 ++ CD 16+和非经典单核细胞CD 14 + CD 16+。然而，很少有人知道单核细胞参与调节免疫反应的患者与gastric malignances.Material和方法：在这项研究中，外周血单核细胞的表型测定胃肿瘤患者的流式细胞术。我们还分析了白细胞介素10（IL-10）和肿瘤坏死因子α（TNF-α）的生产水平后，24小时的刺激外周血单核细胞与脂多糖。在胃癌患者组中获得的结果进行了比较，那些年龄匹配组的健康individuals.Results：我们观察到一个显着的百分比下降单核细胞在胃癌患者的外周血相比，健康供体。此外，与健康志愿者相比，胃癌患者显示出⑶ 14 ++ ⑶ 16-单核细胞亚群的显著减少和⑶ 14 ++ ⑶ 16+和⑶ 14 + ⑶ 16+单核细胞亚群的增加。在胃癌患者中，我们也观察到增加生产的IL-10相比，controls.Conclusions：总之，这项研究表明，胃恶性肿瘤显着调节外周血单核细胞的表型，使他们的抗炎特性。外周血中抗炎单核细胞的治疗靶向可以调节肿瘤环境中巨噬细胞的复杂活性。
Introduction: Gastric cancer is one of the most common malignancies diagnosed worldwide. Despite therapeutic options, prognosis is unfavorable, particularly when dealing with an advanced stage carcinoma. Similarly to other malignancies, the development of gastric cancer is associated with increased macrophage infiltration. Monocytes are peripheral blood precursors of tissue macrophages. In the relation to the expression of CD14 and CD16 receptors, human peripheral blood monocytes can be divided into: "clasical monocytes" CD14++CD16-, intermediate monocytes CD14++CD16+ and non-classical monocytes CD14+CD16+. However, little is known about monocytes involvement in the regulation of immune response in patients with gastric malignancies.Material and methods: In this study, the phenotype of peripheral blood monocytes was determined in patients with gastric tumors by flow cytometry. We also analyzed the level of interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF-alpha) production after 24 h stimulation of peripheral blood mononuclear cells with lipopolysaccharide. The results obtained in group of gastric cancer patients were compared to those of age-matched group of healthy individuals.Results: We observed a significant decrease in percentage of monocytes in the peripheral blood of gastric cancer patients as compared to healthy donors. Moreover gastric cancer patients shows a significant decrease of CD14++CD16- monocytes subpopulation and an increase in CDI4++CD16+ and CD14+CD16+ monocytes subpopulations as compared to healthy volunteers. In stomach cancer patients we also have observed increased production of IL-10 as compared to controls.Conclusions: In summary, this study has revealed that gastric malignancies significantly modulate the phenotype of peripheral blood monocytes giving them anti-inflammatory properties. Therapeutic targeting of anti-inflammatory monocytes in the peripheral blood could modulate the complex activities of macrophages in tumor environment.