Solution structure of Der f 2, the major mite allergen for atopic diseases
Solution structure of Der f 2, the major mite allergen for atopic diseases
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DOI：
10.1074/jbc.273.1.356
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发表时间：
1998-01-02
影响因子：
4.8
通讯作者：
Inagaki, F
中科院分区：
文献类型：
--
作者：
Ichikawa, S;Hatanaka, H;Inagaki, F
室内尘螨会引起严重的过敏性疾病，如哮喘和皮炎。在粉尘螨变应原中，Der f 2在特应性患者中的阳性率最高，但它在尘螨中的生物学功能以及它的同系物在人类和其他动物中的功能一直是完全未知的。我们用多维核磁共振波谱测定了Der-F2的三级结构。Der f2被发现是免疫球蛋白折叠的单域蛋白，其结构与转谷氨酰胺酶的两个调节域的结构最相似，这一事实与细菌表面结合等小片段信息暗示，Her f2与螨类的天然抗菌防御系统有关。在此基础上，对免疫球蛋白E表位进行了讨论。
House dust mites cause heavy atopic diseases such as asthma and dermatitis. Among allergens from Dermatophagoides farinae, Der f 2 shows the highest positive rate for atopic patients, but its biological function in mites has been perfectly unknown, as well as the functions of its homologs in human and other animals. We have determined the tertiary structure of Der f 2 by multidimensional nuclear magnetic resonance spectroscopy. Der f 2 was found to be a single-domain protein of immunoglobulin fold, and its structure was the most similar to those of the two regulatory domains of transglutaminase, This fact, binding to the bacterial surface, and other small pieces of information hinted that Her f 2 is related to the innate antibacterial defense system in mites. The immunoglobulin E epitopes are also discussed on the basis of the tertiary structure.