Effect of bone marrow-derived mononuclear cells on nerve regeneration in the transection model of the rat sciatic nerve
Effect of bone marrow-derived mononuclear cells on nerve regeneration in the transection model of the rat sciatic nerve
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DOI：
10.1016/j.jocn.2009.01.031
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发表时间：
2009-09-01
影响因子：
2
通讯作者：
Srivastava, Arti
中科院分区：
文献类型：
--
作者：
Goel, Rohit Kumar;Suri, Vaishali;Srivastava, Arti
骨髓源性干细胞可提高神经损伤、脊髓损伤和脑梗死的再生速度和临床改善。最近在大鼠脊髓脱髓鞘的实验表明，髓鞘再生是特定的骨髓来源的单核细胞（BM-MNC）部分的静脉内输送，虽然这部分在外周神经再生的具体作用还没有被检查。因此，我们在大鼠坐骨神经横断模型中评估BM-MNCs在周围神经再生中的作用。麻醉后，在手术显微镜下切断20只成年雄性Wistar大鼠的右侧坐骨神经。在实验组中，用两条神经外膜微缝合线对合神经的切断端，用大鼠BM-MNCs填充差距，并用纤维蛋白胶覆盖对合的神经末端。在对照组中，仅用两条神经外膜显微缝合线和纤维蛋白封闭剂修复横断的神经末端。分别于术后30 d和60 d进行神经组织学检查，比较两组神经再生情况。神经吻合后30天和60天，试验组的恢复情况都要好得多。在距离神经修复部位5 mm和10 mm处的轴突再生、髓鞘再生和髓鞘厚度存在统计学显著差异。雪旺细胞增殖和退行性变化在对照组中更为普遍。这项研究表明，BM-MNCs（可以很容易地从骨髓抽吸物中分离）局部递送到受损的外周神经中增加了神经再生的速度和程度。本研究强调了BM-MNCs在周围神经再生中的作用。(C)2009爱思唯尔有限公司保留所有权利。
Bone marrow-derived stem cells enhance the rate of regeneration and clinical improvement in nerve injury, spinal cord injury and brain infarction. Recent experiments in rat spinal cord demyelination showed that remyelination was specific to intravenous delivery of the bone marrow-derived mononuclear cell (BM-MNC) fraction, although the specific role of this fraction in peripheral nerve regeneration has not been examined. Therefore we evaluated the role of BM-MNCs in peripheral nerve regeneration in the rat sciatic nerve transection model. After anesthesia, the right sciatic nerve of 20 adult-male Wistar rats was transected under an operating microscope. In the test group, the cut ends of the nerve were approximated with two epineural microsutures, the gap was filled with rat BM-MNCs and the approximated nerve ends were covered with fibrin glue. In the control group, the transected nerve ends were repaired with two epineural microsutures and fibrin sealant only. Histological assessment of the nerve was performed 30 days and 60 days after the operation and regenerative changes were compared between the two groups. The recovery after nerve anastamosis was far better in the test group at both 30 days and 60 days. There was a statistically significant difference in axonal regeneration, remyelination and myelin thickness at sites 5 mm and 10 mm from the site of repair of the nerve. Schwann cell proliferation and degenerative changes were more prevalent in the controls. This study demonstrates that local delivery of BM-MNCs (which can be isolated easily from bone marrow aspirates) into injured peripheral nerve increases the rate and degree of nerve regeneration. The present study highlights the role of BM-MNCs in peripheral nerve regeneration. (C) 2009 Elsevier Ltd. All rights reserved.